1xse

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[[Image:1xse.gif|left|200px]]
 
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==Crystal Structure of Guinea Pig 11beta-Hydroxysteroid Dehydrogenase Type 1==
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The line below this paragraph, containing "STRUCTURE_1xse", creates the "Structure Box" on the page.
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<StructureSection load='1xse' size='340' side='right'caption='[[1xse]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1xse]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cavia_porcellus Cavia porcellus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XSE FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>
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{{STRUCTURE_1xse| PDB=1xse | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xse OCA], [https://pdbe.org/1xse PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xse RCSB], [https://www.ebi.ac.uk/pdbsum/1xse PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xse ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DHI1_CAVPO DHI1_CAVPO] Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone. Catalyzes reversibly the conversion of 7-ketocholesterol to 7-beta-hydroxycholesterol. In intact cells, the reaction runs only in one direction, from 7-ketocholesterol to 7-beta-hydroxycholesterol (By similarity).<ref>PMID:10699594</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xs/1xse_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xse ConSurf].
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<div style="clear:both"></div>
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'''Crystal Structure of Guinea Pig 11beta-Hydroxysteroid Dehydrogenase Type 1'''
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==See Also==
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*[[Hydroxysteroid dehydrogenase 3D structures|Hydroxysteroid dehydrogenase 3D structures]]
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== References ==
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==Overview==
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<references/>
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The metabolic reduction of 11-keto groups in glucocorticoid steroids such as cortisone leads to the nuclear receptor ligand cortisol. This conversion is an example of pre-receptor regulation and constitutes a novel pharmacological target for the treatment of metabolic disorders such as insulin resistance and possibly other derangements observed in the metabolic syndrome, such as hyperlipidemia, hypertension, and lowered insulin secretion. This reaction is carried out by the NADPH-dependent type 1 11beta-hydroxysteroid dehydrogenase (11beta-HSD1), an enzyme attached through an integral N-terminal transmembrane helix to the lipid bilayer and located with its active site within the lumen of the endoplasmic reticulum. Here we report the crystal structure of recombinant guinea pig 11beta-HSD1. This variant was determined in complex with NADP at 2.5 A resolution and crystallized in the presence of detergent and guanidinium hydrochloride. The overall structure of guinea pig 11beta-HSD1 shows a clear relationship to other members of the superfamily of short-chain dehydrogenases/reductases but harbors a unique C-terminal helical segment that fulfills three essential functions and accordingly is involved in subunit interactions, contributes to active site architecture, and is necessary for lipid-membrane interactions. The structure provides a model for enzyme-lipid bilayer interactions and suggests a funneling of lipophilic substrates such as steroid hormones from the hydrophobic membrane environment to the enzyme active site.
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__TOC__
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</StructureSection>
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==About this Structure==
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1XSE is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Cavia_porcellus Cavia porcellus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSE OCA].
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==Reference==
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The crystal structure of guinea pig 11beta-hydroxysteroid dehydrogenase type 1 provides a model for enzyme-lipid bilayer interactions., Ogg D, Elleby B, Norstrom C, Stefansson K, Abrahmsen L, Oppermann U, Svensson S, J Biol Chem. 2005 Feb 4;280(5):3789-94. Epub 2004 Nov 12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15542590 15542590]
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[[Category: 11-beta-hydroxysteroid dehydrogenase]]
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[[Category: Cavia porcellus]]
[[Category: Cavia porcellus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Abrahmsen, L.]]
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[[Category: Abrahmsen L]]
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[[Category: Elleby, B.]]
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[[Category: Elleby B]]
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[[Category: Norstrom, C.]]
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[[Category: Norstrom C]]
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[[Category: Ogg, D.]]
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[[Category: Ogg D]]
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[[Category: Oppermann, U.]]
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[[Category: Oppermann U]]
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[[Category: Stefansson, K.]]
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[[Category: Stefansson K]]
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[[Category: Svensson, S.]]
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[[Category: Svensson S]]
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[[Category: 11beta-hydroxysteroid dehydrogenase dimer]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:27:12 2008''
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Current revision

Crystal Structure of Guinea Pig 11beta-Hydroxysteroid Dehydrogenase Type 1

PDB ID 1xse

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