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| - | [[Image:1xta.gif|left|200px]] | |
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| - | <!--
| + | ==Crystal Structure of Natrin, a snake venom CRISP from Taiwan cobra (Naja atra)== |
| - | The line below this paragraph, containing "STRUCTURE_1xta", creates the "Structure Box" on the page.
| + | <StructureSection load='1xta' size='340' side='right'caption='[[1xta]], [[Resolution|resolution]] 1.58Å' scene=''> |
| - | You may change the PDB parameter (which sets the PDB file loaded into the applet)
| + | == Structural highlights == |
| - | or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| + | <table><tr><td colspan='2'>[[1xta]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Naja_atra Naja atra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XTA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XTA FirstGlance]. <br> |
| - | or leave the SCENE parameter empty for the default display.
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.58Å</td></tr> |
| - | -->
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xta FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xta OCA], [https://pdbe.org/1xta PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xta RCSB], [https://www.ebi.ac.uk/pdbsum/1xta PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xta ProSAT]</span></td></tr> |
| - | {{STRUCTURE_1xta| PDB=1xta | SCENE= }}
| + | </table> |
| - | | + | == Function == |
| - | '''Crystal Structure of Natrin, a snake venom CRISP from Taiwan cobra (Naja atra)'''
| + | [https://www.uniprot.org/uniprot/CRVP1_NAJAT CRVP1_NAJAT] Inhibits calcium-activated potassium channels (KCa1.1/KCNMA1), voltage-gated potassium channel Kv1.3/KCNA3, and the calcium release channel/ryanodine receptor (RyR). Binds specifically to type 1 RyR (RyR1) from skeletal muscle. Inhibit both the binding of ryanodine to RyR1, and RyR1's calcium-channel activity. Inhibits carbachol-induced muscle contraction and weakly blocks muscle contraction evoked by potassium.<ref>PMID:15581679</ref> <ref>PMID:17070778</ref> <ref>PMID:18658224</ref> <ref>PMID:16042391</ref> |
| - | | + | == Evolutionary Conservation == |
| - | | + | [[Image:Consurf_key_small.gif|200px|right]] |
| - | ==Overview== | + | Check<jmol> |
| - | Cysteine-rich secretory proteins (CRISPs) play an important role in the innate immune system and are transcriptionally regulated by androgens in several tissues. The proteins are mostly found in the epididymis and granules of mammals, whilst a number of snake venoms also contain CRISP-family proteins. The natrin protein from the venom of Naja atra (Taiwan cobra), which belongs to a family of CRISPs and has a cysteine-rich C-terminal amino-acid sequence, has been purified using a three-stage chromatography procedure and crystals suitable for X-ray analysis have been obtained using the hanging-drop vapour-diffusion method. X-ray diffraction data were collected to 1.58 A resolution using synchrotron radiation; the crystals belong to space group C222(1), with unit-cell parameters a = 59.172, b = 65.038, c = 243.156 A. There are two protein molecules in the asymmetric unit and the Matthews coefficient is estimated to be 2.35 A3 Da(-1), corresponding to a solvent content of 47.60%.
| + | <jmolCheckbox> |
| - | | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xt/1xta_consurf.spt"</scriptWhenChecked> |
| - | ==About this Structure== | + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| - | 1XTA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Naja_atra Naja atra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XTA OCA].
| + | <text>to colour the structure by Evolutionary Conservation</text> |
| - | | + | </jmolCheckbox> |
| - | ==Reference== | + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xta ConSurf]. |
| - | Purification, crystallization and preliminary X-ray crystallographic analysis of a cysteine-rich secretory protein (CRISP) from Naja atra venom., Wang YL, Goh KX, Wu WG, Chen CJ, Acta Crystallogr D Biol Crystallogr. 2004 Oct;60(Pt 10):1912-5. Epub 2004, Sep 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15388950 15388950]
| + | <div style="clear:both"></div> |
| - | [[Category: Naja atra]] | + | == References == |
| - | [[Category: Single protein]]
| + | <references/> |
| - | [[Category: Chen, C J.]] | + | __TOC__ |
| - | [[Category: Goh, K X.]] | + | </StructureSection> |
| - | [[Category: Huang, W N.]] | + | [[Category: Large Structures]] |
| - | [[Category: Lee, S C.]]
| + | |
| - | [[Category: Wang, Y L.]] | + | |
| - | [[Category: Wu, W G.]] | + | |
| - | [[Category: Cobra]] | + | |
| - | [[Category: Crisp]] | + | |
| - | [[Category: Ion channel blocking]] | + | |
| | [[Category: Naja atra]] | | [[Category: Naja atra]] |
| - | [[Category: Natrin]] | + | [[Category: Chen C-J]] |
| - | [[Category: Serine protease]] | + | [[Category: Goh K-X]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:29:07 2008''
| + | [[Category: Huang W-N]] |
| | + | [[Category: Lee S-C]] |
| | + | [[Category: Wang Y-L]] |
| | + | [[Category: Wu W-G]] |
| Structural highlights
Function
CRVP1_NAJAT Inhibits calcium-activated potassium channels (KCa1.1/KCNMA1), voltage-gated potassium channel Kv1.3/KCNA3, and the calcium release channel/ryanodine receptor (RyR). Binds specifically to type 1 RyR (RyR1) from skeletal muscle. Inhibit both the binding of ryanodine to RyR1, and RyR1's calcium-channel activity. Inhibits carbachol-induced muscle contraction and weakly blocks muscle contraction evoked by potassium.[1] [2] [3] [4]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
References
- ↑ Chang LS, Liou JC, Lin SR, Cheng YC. Purification and characterization of Taiwan cobra venom proteins with weak toxicity. Toxicon. 2005 Jan;45(1):21-5. PMID:15581679 doi:10.1016/j.toxicon.2004.09.002
- ↑ Wang F, Li H, Liu MN, Song H, Han HM, Wang QL, Yin CC, Zhou YC, Qi Z, Shu YY, Lin ZJ, Jiang T. Structural and functional analysis of natrin, a venom protein that targets various ion channels. Biochem Biophys Res Commun. 2006 Dec 15;351(2):443-8. Epub 2006 Oct 20. PMID:17070778 doi:10.1016/j.bbrc.2006.10.067
- ↑ Zhou Q, Wang QL, Meng X, Shu Y, Jiang T, Wagenknecht T, Yin CC, Sui SF, Liu Z. Structural and functional characterization of ryanodine receptor-natrin toxin interaction. Biophys J. 2008 Nov 1;95(9):4289-99. doi: 10.1529/biophysj.108.137224. Epub 2008 , Jul 25. PMID:18658224 doi:10.1529/biophysj.108.137224
- ↑ Wang J, Shen B, Guo M, Lou X, Duan Y, Cheng XP, Teng M, Niu L, Liu Q, Huang Q, Hao Q. Blocking effect and crystal structure of natrin toxin, a cysteine-rich secretory protein from Naja atra venom that targets the BKCa channel. Biochemistry. 2005 Aug 2;44(30):10145-52. PMID:16042391 doi:10.1021/bi050614m
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