2b3o

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(New page: 200px<br /> <applet load="2b3o" size="450" color="white" frame="true" align="right" spinBox="true" caption="2b3o, resolution 2.8&Aring;" /> '''Crystal structure of...)
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[[Image:2b3o.gif|left|200px]]<br />
 
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<applet load="2b3o" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2b3o, resolution 2.8&Aring;" />
 
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'''Crystal structure of human tyrosine phosphatase SHP-1'''<br />
 
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==Overview==
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==Crystal structure of human tyrosine phosphatase SHP-1==
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SHP-1 is a cytosolic protein-tyrosine phosphatase that behaves as a, negative regulator in eukaryotic cellular signaling pathways. To, understand its regulatory mechanism, we have determined the crystal, structure of the C-terminal truncated human SHP-1 in the inactive, conformation at 2.8-A resolution and refined the structure to a, crystallographic R-factor of 24.0%. The three-dimensional structure shows, that the ligand-free SHP-1 has an auto-inhibited conformation. Its N-SH2, domain blocks the catalytic domain and keeps the enzyme in the inactive, conformation, which supports that the phosphatase activity of SHP-1 is, primarily regulated by the N-SH2 domain. In addition, the C-SH2 domain of, SHP-1 has a different orientation from and is more flexible than that of, SHP-2, which enables us to propose an enzymatic activation mechanism in, which the C-SH2 domains of SHPs could be involved in searching for, phosphotyrosine activators.
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<StructureSection load='2b3o' size='340' side='right'caption='[[2b3o]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2b3o]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B3O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2B3O FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b3o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b3o OCA], [https://pdbe.org/2b3o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b3o RCSB], [https://www.ebi.ac.uk/pdbsum/2b3o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b3o ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PTN6_HUMAN PTN6_HUMAN] Modulates signaling by tyrosine phosphorylated cell surface receptors such as KIT and the EGF receptor/EGFR. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation. Plays a key role in hematopoiesis.<ref>PMID:11266449</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b3/2b3o_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2b3o ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2B3O is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Active as [http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2B3O OCA].
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*[[Tyrosine phosphatase 3D structures|Tyrosine phosphatase 3D structures]]
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== References ==
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==Reference==
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<references/>
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Crystal structure of human protein-tyrosine phosphatase SHP-1., Yang J, Liu L, He D, Song X, Liang X, Zhao ZJ, Zhou GW, J Biol Chem. 2003 Feb 21;278(8):6516-20. Epub 2002 Dec 13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12482860 12482860]
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Protein-tyrosine-phosphatase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: He D]]
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[[Category: He, D.]]
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[[Category: Liang X]]
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[[Category: Liang, X.]]
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[[Category: Liu L]]
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[[Category: Liu, L.]]
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[[Category: Song X]]
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[[Category: Song, X.]]
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[[Category: Yang J]]
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[[Category: Yang, J.]]
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[[Category: Zhao ZJ]]
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[[Category: Zhao, Z.J.]]
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[[Category: Zhou GW]]
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[[Category: Zhou, G.W.]]
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[[Category: protein tyrosine phosphatase]]
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[[Category: shp-1]]
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[[Category: signaling]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 20:58:00 2007''
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Current revision

Crystal structure of human tyrosine phosphatase SHP-1

PDB ID 2b3o

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