1y66

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:55, 14 February 2024) (edit) (undo)
 
(10 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1y66.gif|left|200px]]
 
-
<!--
+
==Dioxane contributes to the altered conformation and oligomerization state of a designed engrailed homeodomain variant==
-
The line below this paragraph, containing "STRUCTURE_1y66", creates the "Structure Box" on the page.
+
<StructureSection load='1y66' size='340' side='right'caption='[[1y66]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[1y66]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y66 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Y66 FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=DIO:1,4-DIETHYLENE+DIOXIDE'>DIO</scene></td></tr>
-
{{STRUCTURE_1y66| PDB=1y66 | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1y66 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y66 OCA], [https://pdbe.org/1y66 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1y66 RCSB], [https://www.ebi.ac.uk/pdbsum/1y66 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1y66 ProSAT]</span></td></tr>
-
 
+
</table>
-
'''Dioxane contributes to the altered conformation and oligomerization state of a designed engrailed homeodomain variant'''
+
__TOC__
-
 
+
</StructureSection>
-
 
+
[[Category: Escherichia coli]]
-
==Overview==
+
[[Category: Large Structures]]
-
Our goal was to compute a stable, full-sequence design of the Drosophila melanogaster engrailed homeodomain. Thermal and chemical denaturation data indicated the design was significantly more stable than was the wild-type protein. The data were also nearly identical to those for a similar, later full-sequence design, which was shown by NMR to adopt the homeodomain fold: a three-helix, globular monomer. However, a 1.65 A crystal structure of the design described here turned out to be of a completely different fold: a four-helix, rodlike tetramer. The crystallization conditions included approximately 25% dioxane, and subsequent experiments by circular dichroism and sedimentation velocity analytical ultracentrifugation indicated that dioxane increases the helicity and oligomerization state of the designed protein. We attribute at least part of the discrepancy between the target fold and the crystal structure to the presence of a high concentration of dioxane.
+
[[Category: Hom GK]]
-
 
+
[[Category: Lassila JK]]
-
==About this Structure==
+
[[Category: Mayo SL]]
-
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y66 OCA].
+
[[Category: Thomas LM]]
-
 
+
-
==Reference==
+
-
Dioxane contributes to the altered conformation and oligomerization state of a designed engrailed homeodomain variant., Hom GK, Lassila JK, Thomas LM, Mayo SL, Protein Sci. 2005 Apr;14(4):1115-9. Epub 2005 Mar 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15741348 15741348]
+
-
[[Category: Hom, G K.]]
+
-
[[Category: Lassila, J K.]]
+
-
[[Category: Mayo, S L.]]
+
-
[[Category: Thomas, L M.]]
+
-
[[Category: Dioxane]]
+
-
[[Category: Engrailed homeodomain]]
+
-
[[Category: Protein design]]
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:55:09 2008''
+

Current revision

Dioxane contributes to the altered conformation and oligomerization state of a designed engrailed homeodomain variant

PDB ID 1y66

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1y66"
Personal tools