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1y7y

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[[Image:1y7y.gif|left|200px]]
 
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==High-resolution crystal structure of the restriction-modification controller protein C.AhdI from Aeromonas hydrophila==
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The line below this paragraph, containing "STRUCTURE_1y7y", creates the "Structure Box" on the page.
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<StructureSection load='1y7y' size='340' side='right'caption='[[1y7y]], [[Resolution|resolution]] 1.69&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1y7y]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aeromonas_hydrophila Aeromonas hydrophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y7Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Y7Y FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.69&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1y7y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y7y OCA], [https://pdbe.org/1y7y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1y7y RCSB], [https://www.ebi.ac.uk/pdbsum/1y7y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1y7y ProSAT]</span></td></tr>
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{{STRUCTURE_1y7y| PDB=1y7y | SCENE= }}
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</table>
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== Function ==
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'''High-resolution crystal structure of the restriction-modification controller protein C.AhdI from Aeromonas hydrophila'''
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[https://www.uniprot.org/uniprot/Q7X0F0_AERHY Q7X0F0_AERHY]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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Restriction-modification (R-M) systems serve to protect the host bacterium from invading bacteriophage. The multi-component system includes a methyltransferase, which recognizes and methylates a specific DNA sequence, and an endonuclease which recognises the same sequence and cleaves within or close to this site. The endonuclease will only cleave DNA that is unmethylated at the specific site, thus host DNA is protected while non-host DNA is cleaved. However, following DNA replication, expression of the endonuclease must be delayed until the host DNA is appropriately methylated. In many R-M systems, this regulation is achieved at the transcriptional level via the controller protein, or C-protein. We have solved the first X-ray structure of an R-M controller protein, C.AhdI, to 1.69 A resolution using selenomethionine MAD. C.AhdI is part of a Type IIH R-M system from the pathogen Aeromonas hydrophila. The structure reveals an all-alpha protein that contains a classical helix-turn-helix (HTH) domain and can be assigned to the Xre family of transcriptional regulators. Unlike its monomeric structural homologues, an extended helix generates an interface that results in dimerisation of the free protein. The dimer is electrostatically polarised and a positively charged surface corresponds to the position of the DNA recognition helices of the HTH domain. Comparison with the structure of the lambda cI ternary complex suggests that C.AhdI activates transcription through direct contact with the sigma70 subunit of RNA polymerase.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/y7/1y7y_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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1Y7Y is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aeromonas_hydrophila Aeromonas hydrophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y7Y OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1y7y ConSurf].
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High-resolution crystal structure of the restriction-modification controller protein C.AhdI from Aeromonas hydrophila., McGeehan JE, Streeter SD, Papapanagiotou I, Fox GC, Kneale GG, J Mol Biol. 2005 Feb 25;346(3):689-701. Epub 2005 Jan 12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15713456 15713456]
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Aeromonas hydrophila]]
[[Category: Aeromonas hydrophila]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Fox, G C.]]
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[[Category: Fox GC]]
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[[Category: Kneale, G G.]]
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[[Category: Kneale GG]]
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[[Category: McGeehan, J E.]]
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[[Category: McGeehan JE]]
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[[Category: Papapanagiotou, I.]]
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[[Category: Papapanagiotou I]]
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[[Category: Streeter, S D.]]
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[[Category: Streeter SD]]
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[[Category: Dna-binding protein]]
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[[Category: Helix-turn-helix]]
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[[Category: Transcriptional regulator]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:58:57 2008''
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Current revision

High-resolution crystal structure of the restriction-modification controller protein C.AhdI from Aeromonas hydrophila

PDB ID 1y7y

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