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| - | [[Image:1yh0.gif|left|200px]] | + | #REDIRECT [[3kzc]] This PDB entry is obsolete and replaced by 3kzc |
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| - | {{STRUCTURE_1yh0| PDB=1yh0 | SCENE= }}
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| - | '''Crystal Structure of Acetylornithine Transcarbamylase'''
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| - | ==Overview==
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| - | We have identified in Xanthomonas campestris a novel N-acetylornithine transcarbamylase that replaces ornithine transcarbamylase in the canonic arginine biosynthetic pathway of several Eubacteria. The crystal structures of the protein in the presence and absence of the reaction product, N-acetylcitrulline, were determined. This new family of transcarbamylases lacks the DxxSMG motif that is characteristic of all ornithine transcarbamylases (OTCases) and contains a novel proline-rich loop that forms part of the active site. The specificity for N-acetylornithine is conferred by hydrogen bonding with residues in the proline-rich loop via water molecules and by hydrophobic interactions with residues from the adjacent 80's, 120's, and proline-rich loops. This novel protein structure provides a starting point for rational design of specific analogs that may be useful in combating human and plant pathogens that utilize acetylornithine transcarbamylase rather than ornithine transcarbamylase.
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| - | ==About this Structure==
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| - | 1YH0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Xanthomonas_campestris Xanthomonas campestris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YH0 OCA].
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| - | ==Reference==
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| - | Crystal structure of N-acetylornithine transcarbamylase from Xanthomonas campestris: a novel enzyme in a new arginine biosynthetic pathway found in several eubacteria., Shi D, Morizono H, Yu X, Roth L, Caldovic L, Allewell NM, Malamy MH, Tuchman M, J Biol Chem. 2005 Apr 15;280(15):14366-9. Epub 2005 Feb 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15731101 15731101]
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| - | [[Category: Single protein]]
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| - | [[Category: Xanthomonas campestris]]
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| - | [[Category: Allewell, N M.]]
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| - | [[Category: Caldovic, L.]]
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| - | [[Category: Malamy, M H.]]
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| - | [[Category: Morizono, H.]]
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| - | [[Category: Roth, L.]]
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| - | [[Category: Shi, D.]]
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| - | [[Category: Tuchman, M.]]
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| - | [[Category: Yu, X.]]
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| - | [[Category: Acetylcitrulline]]
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| - | [[Category: Acetylornithine]]
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| - | [[Category: Argf]]
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| - | [[Category: Transcarbamylase]]
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:18:25 2008''
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