1ywm

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[[Image:1ywm.gif|left|200px]]
 
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==Crystal structure of the N-terminal domain of group B Streptococcus alpha C protein==
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The line below this paragraph, containing "STRUCTURE_1ywm", creates the "Structure Box" on the page.
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<StructureSection load='1ywm' size='340' side='right'caption='[[1ywm]], [[Resolution|resolution]] 1.86&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1ywm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_agalactiae_A909 Streptococcus agalactiae A909]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YWM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YWM FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.86&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DTU:(2R,3S)-1,4-DIMERCAPTOBUTANE-2,3-DIOL'>DTU</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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{{STRUCTURE_1ywm| PDB=1ywm | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ywm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ywm OCA], [https://pdbe.org/1ywm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ywm RCSB], [https://www.ebi.ac.uk/pdbsum/1ywm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ywm ProSAT]</span></td></tr>
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</table>
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'''Crystal structure of the N-terminal domain of group B Streptococcus alpha C protein'''
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== Function ==
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[https://www.uniprot.org/uniprot/BCA_STRA1 BCA_STRA1] May play a role in both virulence and immunity.
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Group B Streptococcus (GBS) is the leading cause of bacterial pneumonia, sepsis, and meningitis among neonates and an important cause of morbidity among pregnant women and immunocompromised adults. Invasive diseases due to GBS are attributed to the ability of the pathogen to translocate across human epithelial surfaces. The alpha C protein (ACP) has been identified as an invasin that plays a role in internalization and translocation of GBS across epithelial cells. The soluble N-terminal domain of ACP (NtACP) blocks the internalization of GBS. We determined the 1.86-A resolution crystal structure of NtACP comprising residues Ser(52) through Leu(225) of the full-length ACP. NtACP has two domains, an N-terminal beta-sandwich and a C-terminal three-helix bundle. Structural and topological alignments reveal that the beta-sandwich shares structural elements with the type III fibronectin fold (FnIII), but includes structural elaborations that make it unique. We have identified a potential integrin-binding motif consisting of Lys-Thr-Asp(146), Arg(110), and Asp(118). A similar arrangement of charged residues has been described in other invasins. ACP shows a heparin binding activity that requires NtACP. We propose a possible heparin-binding site, including one surface of the three-helix bundle, and nearby portions of the sandwich and repeat domains. We have validated this prediction using assays of the heparin binding and cell-adhesion properties of engineered fragments of ACP. This is the first crystal structure of a member of the highly conserved Gram-positive surface alpha-like protein family, and it will enable the internalization mechanism of GBS to be dissected at the atomic level.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/yw/1ywm_consurf.spt"</scriptWhenChecked>
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1YWM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_agalactiae Streptococcus agalactiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YWM OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Crystal structure of the N-terminal domain of the group B streptococcus alpha C protein., Auperin TC, Bolduc GR, Baron MJ, Heroux A, Filman DJ, Madoff LC, Hogle JM, J Biol Chem. 2005 May 6;280(18):18245-52. Epub 2005 Mar 6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15753100 15753100]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ywm ConSurf].
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[[Category: Single protein]]
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<div style="clear:both"></div>
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[[Category: Streptococcus agalactiae]]
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__TOC__
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[[Category: Auperin, T C.]]
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</StructureSection>
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[[Category: Baron, M J.]]
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[[Category: Large Structures]]
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[[Category: Bolduc, G R.]]
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[[Category: Streptococcus agalactiae A909]]
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[[Category: Filman, D J.]]
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[[Category: Auperin TC]]
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[[Category: Heroux, A.]]
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[[Category: Baron MJ]]
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[[Category: Hogle, J M.]]
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[[Category: Bolduc GR]]
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[[Category: Madoff, L C.]]
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[[Category: Filman DJ]]
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[[Category: Antiparallel three-helix bundle]]
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[[Category: Heroux A]]
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[[Category: Beta sandwich]]
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[[Category: Hogle JM]]
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[[Category: Fibronectin fold]]
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[[Category: Madoff LC]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:53:17 2008''
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Current revision

Crystal structure of the N-terminal domain of group B Streptococcus alpha C protein

PDB ID 1ywm

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