1z25

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[[Image:1z25.gif|left|200px]]
 
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==Structure of P.furiosus Argonaute with bound Mn2+==
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The line below this paragraph, containing "STRUCTURE_1z25", creates the "Structure Box" on the page.
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<StructureSection load='1z25' size='340' side='right'caption='[[1z25]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1z25]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z25 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Z25 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
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{{STRUCTURE_1z25| PDB=1z25 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1z25 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z25 OCA], [https://pdbe.org/1z25 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1z25 RCSB], [https://www.ebi.ac.uk/pdbsum/1z25 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1z25 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q8U3D2_PYRFU Q8U3D2_PYRFU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z2/1z25_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1z25 ConSurf].
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<div style="clear:both"></div>
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'''Structure of P.furiosus Argonaute with bound Mn2+'''
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==See Also==
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*[[Argonaute 3D structures|Argonaute 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Genetic, biochemical and structural studies have implicated Argonaute proteins as the catalytic core of the RNAi effector complex, RISC. Here we show that recombinant, human Argonaute2 can combine with a small interfering RNA (siRNA) to form minimal RISC that accurately cleaves substrate RNAs. Recombinant RISC shows many of the properties of RISC purified from human or Drosophila melanogaster cells but also has surprising features. It shows no stimulation by ATP, suggesting that factors promoting product release are missing from the recombinant enzyme. The active site is made up of a unique Asp-Asp-His (DDH) motif. In the RISC reconstitution system, the siRNA 5' phosphate is important for the stability and the fidelity of the complex but is not essential for the creation of an active enzyme. These studies demonstrate that Argonaute proteins catalyze mRNA cleavage within RISC and provide a source of recombinant enzyme for detailed biochemical studies of the RNAi effector complex.
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[[Category: Large Structures]]
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==About this Structure==
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1Z25 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z25 OCA].
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==Reference==
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Purified Argonaute2 and an siRNA form recombinant human RISC., Rivas FV, Tolia NH, Song JJ, Aragon JP, Liu J, Hannon GJ, Joshua-Tor L, Nat Struct Mol Biol. 2005 Apr;12(4):340-9. Epub 2005 Mar 30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15800637 15800637]
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[[Category: Pyrococcus furiosus]]
[[Category: Pyrococcus furiosus]]
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[[Category: Single protein]]
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[[Category: Aragon JP]]
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[[Category: Aragon, J P.]]
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[[Category: Hannon GJ]]
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[[Category: Hannon, G J.]]
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[[Category: Joshua-Tor L]]
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[[Category: Joshua-Tor, L.]]
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[[Category: Liu J]]
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[[Category: Liu, J.]]
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[[Category: Rivas FV]]
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[[Category: Rivas, F V.]]
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[[Category: Song JJ]]
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[[Category: Song, J J.]]
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[[Category: Tolia NH]]
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[[Category: Tolia, N H.]]
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[[Category: Active site]]
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[[Category: Argonaute]]
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[[Category: Mg2+]]
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[[Category: Piwi]]
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[[Category: Rnai]]
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[[Category: Rnaseh]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:05:42 2008''
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Current revision

Structure of P.furiosus Argonaute with bound Mn2+

PDB ID 1z25

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