1z9o

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[[Image:1z9o.gif|left|200px]]
 
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==1.9 Angstrom Crystal Structure of the Rat VAP-A MSP Homology Domain in Complex with the Rat ORP1 FFAT Motif==
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The line below this paragraph, containing "STRUCTURE_1z9o", creates the "Structure Box" on the page.
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<StructureSection load='1z9o' size='340' side='right'caption='[[1z9o]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1z9o]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z9O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Z9O FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1z9o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z9o OCA], [https://pdbe.org/1z9o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1z9o RCSB], [https://www.ebi.ac.uk/pdbsum/1z9o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1z9o ProSAT]</span></td></tr>
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{{STRUCTURE_1z9o| PDB=1z9o | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/VAPA_RAT VAPA_RAT] May play a role in vesicle trafficking.<ref>PMID:19289470</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z9/1z9o_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1z9o ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The FFAT motif is a targeting signal responsible for localizing a number of proteins to the cytosolic surface of the endoplasmic reticulum (ER) and to the nuclear membrane. FFAT motifs bind to members of the highly conserved VAP protein family, which are tethered to the cytoplasmic face of the ER by a C-terminal transmembrane domain. We have solved crystal structures of the rat VAP-A MSP homology domain alone and in complex with an FFAT motif. The co-crystal structure was used to design a VAP mutant that disrupts rat and yeast VAP-FFAT interactions in vitro. The FFAT binding-defective mutant also blocked function of the VAP homolog Scs2p in yeast. Finally, overexpression of the FFAT binding-defective VAP in COS7 cells dramatically altered ER morphology. Our data establish the structural basis of FFAT-mediated ER targeting and suggest that FFAT-targeted proteins play an important role in determining ER morphology.
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'''1.9 Angstrom Crystal Structure of the Rat VAP-A MSP Homology Domain in Complex with the Rat ORP1 FFAT Motif'''
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Structural basis of FFAT motif-mediated ER targeting.,Kaiser SE, Brickner JH, Reilein AR, Fenn TD, Walter P, Brunger AT Structure. 2005 Jul;13(7):1035-45. PMID:16004875<ref>PMID:16004875</ref>
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==Overview==
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The FFAT motif is a targeting signal responsible for localizing a number of proteins to the cytosolic surface of the endoplasmic reticulum (ER) and to the nuclear membrane. FFAT motifs bind to members of the highly conserved VAP protein family, which are tethered to the cytoplasmic face of the ER by a C-terminal transmembrane domain. We have solved crystal structures of the rat VAP-A MSP homology domain alone and in complex with an FFAT motif. The co-crystal structure was used to design a VAP mutant that disrupts rat and yeast VAP-FFAT interactions in vitro. The FFAT binding-defective mutant also blocked function of the VAP homolog Scs2p in yeast. Finally, overexpression of the FFAT binding-defective VAP in COS7 cells dramatically altered ER morphology. Our data establish the structural basis of FFAT-mediated ER targeting and suggest that FFAT-targeted proteins play an important role in determining ER morphology.
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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1Z9O is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z9O OCA].
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</div>
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<div class="pdbe-citations 1z9o" style="background-color:#fffaf0;"></div>
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==Reference==
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==See Also==
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Structural basis of FFAT motif-mediated ER targeting., Kaiser SE, Brickner JH, Reilein AR, Fenn TD, Walter P, Brunger AT, Structure. 2005 Jul;13(7):1035-45. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16004875 16004875]
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*[[Vesicle-associated membrane protein|Vesicle-associated membrane protein]]
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[[Category: Protein complex]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
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[[Category: Brickner, J H.]]
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[[Category: Brickner JH]]
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[[Category: Brunger, A T.]]
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[[Category: Brunger AT]]
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[[Category: Fenn, T D.]]
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[[Category: Fenn TD]]
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[[Category: Kaiser, S E.]]
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[[Category: Kaiser SE]]
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[[Category: Reilein, A R.]]
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[[Category: Reilein AR]]
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[[Category: Walter, P.]]
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[[Category: Walter P]]
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[[Category: Endoplasmic reticulum]]
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[[Category: Er]]
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[[Category: Erg30]]
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[[Category: Ffat motif]]
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[[Category: Immunoglobulin-like beta sheet]]
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[[Category: Msp homology domain]]
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[[Category: Orp]]
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[[Category: Osbp]]
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[[Category: Oxysterol binding protein]]
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[[Category: Scs2]]
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[[Category: Targeting]]
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[[Category: Vap-33]]
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[[Category: Vap-33a]]
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[[Category: Vap-a]]
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[[Category: Vap-b]]
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[[Category: Vap-c]]
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[[Category: Vapa]]
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[[Category: Vapb]]
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[[Category: Vapc]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:21:23 2008''
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Current revision

1.9 Angstrom Crystal Structure of the Rat VAP-A MSP Homology Domain in Complex with the Rat ORP1 FFAT Motif

PDB ID 1z9o

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