1zat

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[[Image:1zat.gif|left|200px]]
 
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==Crystal Structure of an Enterococcus faecium peptidoglycan binding protein at 2.4 A resolution==
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The line below this paragraph, containing "STRUCTURE_1zat", creates the "Structure Box" on the page.
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<StructureSection load='1zat' size='340' side='right'caption='[[1zat]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1zat]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_faecium Enterococcus faecium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZAT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZAT FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_1zat| PDB=1zat | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zat OCA], [https://pdbe.org/1zat PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zat RCSB], [https://www.ebi.ac.uk/pdbsum/1zat PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zat ProSAT]</span></td></tr>
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</table>
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'''Crystal Structure of an Enterococcus faecium peptidoglycan binding protein at 2.4 A resolution'''
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== Function ==
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[https://www.uniprot.org/uniprot/Q3Y185_ENTFD Q3Y185_ENTFD]
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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During the final stages of cell-wall synthesis in bacteria, penicillin-binding proteins (PBPs) catalyse the cross-linking of peptide chains from adjacent glycan strands of nascent peptidoglycan. We have recently shown that this step can be bypassed by an L,D-transpeptidase, which confers high-level beta-lactam-resistance in Enterococcus faecium. The resistance bypass leads to replacement of D-Ala4--&gt;D-Asx-L-Lys3 cross-links generated by the PBPs by L-Lys3--&gt;D-Asx-L-Lys3 cross-links generated by the L,D-transpeptidase. As the first structure of a member of this new transpeptidase family, we have determined the crystal structure of a fragment of the L,D-transpeptidase from E.faecium (Ldt(fm217)) at 2.4A resolution. Ldt(fm217) consists of two domains, the N-terminal domain, a new mixed alpha-beta fold, and the ErfK_YbiS_YhnG C-terminal domain, a representative of the mainly beta class of protein structures. Residue Cys442 of the C-terminal domain has been proposed to be the catalytic residue implicated in the cleavage of the L-Lys-D-Ala peptide bond. Surface analysis of Ldt(fm217) reveals that residue Cys442 is localized in a buried pocket and is accessible by two paths on different sides of the protein. We propose that the two paths to the catalytic residue Cys442 are the binding sites for the acceptor and donor substrates of the L,D-transpeptidase.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/za/1zat_consurf.spt"</scriptWhenChecked>
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1ZAT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecium Enterococcus faecium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZAT OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Crystal structure of a novel beta-lactam-insensitive peptidoglycan transpeptidase., Biarrotte-Sorin S, Hugonnet JE, Delfosse V, Mainardi JL, Gutmann L, Arthur M, Mayer C, J Mol Biol. 2006 Jun 9;359(3):533-8. Epub 2006 Mar 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16647082 16647082]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zat ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Enterococcus faecium]]
[[Category: Enterococcus faecium]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Arthur, M.]]
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[[Category: Arthur M]]
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[[Category: Biarrotte-Sorin, S.]]
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[[Category: Biarrotte-Sorin S]]
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[[Category: Gutmann, L.]]
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[[Category: Gutmann L]]
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[[Category: Hugonnet, J E.]]
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[[Category: Hugonnet J-E]]
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[[Category: Mainardi, J L.]]
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[[Category: Mainardi J-L]]
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[[Category: Mayer, C.]]
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[[Category: Mayer C]]
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[[Category: Rice, L.]]
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[[Category: Rice L]]
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[[Category: Antibiotic resistance]]
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[[Category: Beta-lactam insensitive transpeptidase]]
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[[Category: L,d-transpeptidation]]
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[[Category: Peptidoglycan]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:23:53 2008''
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Current revision

Crystal Structure of an Enterococcus faecium peptidoglycan binding protein at 2.4 A resolution

PDB ID 1zat

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