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| - | [[Image:1zev.gif|left|200px]] | |
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| - | <!--
| + | ==Crystal Structure of a Pathogenic RNA: CUG Repeats== |
| - | The line below this paragraph, containing "STRUCTURE_1zev", creates the "Structure Box" on the page.
| + | <StructureSection load='1zev' size='340' side='right'caption='[[1zev]], [[Resolution|resolution]] 1.58Å' scene=''> |
| - | You may change the PDB parameter (which sets the PDB file loaded into the applet)
| + | == Structural highlights == |
| - | or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| + | <table><tr><td colspan='2'>[[1zev]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZEV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZEV FirstGlance]. <br> |
| - | or leave the SCENE parameter empty for the default display.
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.58Å</td></tr> |
| - | -->
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zev FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zev OCA], [https://pdbe.org/1zev PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zev RCSB], [https://www.ebi.ac.uk/pdbsum/1zev PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zev ProSAT]</span></td></tr> |
| - | {{STRUCTURE_1zev| PDB=1zev | SCENE= }}
| + | </table> |
| - | | + | __TOC__ |
| - | '''Crystal Structure of a Pathogenic RNA: CUG Repeats'''
| + | </StructureSection> |
| - | | + | [[Category: Large Structures]] |
| - | | + | [[Category: Berglund JA]] |
| - | ==Overview== | + | [[Category: Logue JS]] |
| - | Myotonic dystrophy (DM) type 1 is associated with an expansion of (>50) CTG repeats within the 3' untranslated region (UTR) of the dystrophin myotonin protein kinase gene (dmpk). In the corresponding mRNA transcript, the CUG repeats form an extended stem-loop structure. The double-stranded RNA of the stem sequesters RNA binding proteins away from their normal cellular targets resulting in aberrant transcription, alternative splicing patterns, or both, thereby leading to DM. To better understand the structural basis of DM type 1, we determined to 1.58-A resolution the x-ray crystal structure of an 18-bp RNA containing six CUG repeats. The CUG repeats form antiparallel double-stranded helices that stack end-on-end in the crystal to form infinite, pseudocontinuous helices similar to the long CUG stem loops formed by the expanded CUG repeats in DM type 1. The CUG helix is very similar in structure to A-form RNA with the exception of the unique U-U mismatches. This structure provides a high-resolution view of a toxic, trinucleotide repeat RNA.
| + | [[Category: Mooers BH]] |
| - | | + | |
| - | ==About this Structure== | + | |
| - | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZEV OCA]. | + | |
| - | | + | |
| - | ==Reference== | + | |
| - | The structural basis of myotonic dystrophy from the crystal structure of CUG repeats., Mooers BH, Logue JS, Berglund JA, Proc Natl Acad Sci U S A. 2005 Nov 15;102(46):16626-31. Epub 2005 Nov 3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16269545 16269545]
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| - | [[Category: Berglund, J A.]] | + | |
| - | [[Category: Logue, J S.]] | + | |
| - | [[Category: Mooers, B H.]]
| + | |
| - | [[Category: Causitive agent of myotonic dystrophy type i]] | + | |
| - | [[Category: Triplet repeat]] | + | |
| - | [[Category: Uu mismatch]] | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:32:15 2008''
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