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| - | [[Image:1zfh.gif|left|200px]] | |
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| - | <!--
| + | ==TTA Duplex B-DNA== |
| - | The line below this paragraph, containing "STRUCTURE_1zfh", creates the "Structure Box" on the page.
| + | <StructureSection load='1zfh' size='340' side='right'caption='[[1zfh]], [[Resolution|resolution]] 2.51Å' scene=''> |
| - | You may change the PDB parameter (which sets the PDB file loaded into the applet)
| + | == Structural highlights == |
| - | or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| + | <table><tr><td colspan='2'>[[1zfh]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZFH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZFH FirstGlance]. <br> |
| - | or leave the SCENE parameter empty for the default display.
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.51Å</td></tr> |
| - | -->
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zfh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zfh OCA], [https://pdbe.org/1zfh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zfh RCSB], [https://www.ebi.ac.uk/pdbsum/1zfh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zfh ProSAT]</span></td></tr> |
| - | {{STRUCTURE_1zfh| PDB=1zfh | SCENE= }}
| + | </table> |
| - | | + | __TOC__ |
| - | '''TTA Duplex B-DNA'''
| + | </StructureSection> |
| - | | + | [[Category: Large Structures]] |
| - | | + | [[Category: Cavaliere E]] |
| - | ==Overview== | + | [[Category: Harms M]] |
| - | The fundamental question of how sequence defines conformation is explicitly answered if the structures of all possible sequences of a macromolecule are determined. We present here a crystallographic screen of all permutations of the inverted repeat DNA sequence d(CCnnnN6N7N8GG), where N6, N7, and N8 are any of the four naturally occurring nucleotides. At this point, 63 of the 64 possible permutations have been crystallized from a defined set of solutions. When combined with previous work, we have assembled a data set of 37 single-crystal structures from 29 of the sequences in this motif, representing three structural classes of DNA (B-DNA, A-DNA, and four-stranded Holliday junctions). This data set includes a unique set of amphimorphic sequence, those that crystallize in two different conformations and serve to bridge the three structural phases. We have thus constructed a map of DNA structures that can be walked through in single nucleotide steps. Finally, the resulting data set allows us to dissect in detail the stabilization of and conformational variations within structural classes and identify significant conformational deviations within a particular structural class that result from sequence rather than crystal or crystallization effects.
| + | [[Category: Hays FA]] |
| - | | + | [[Category: Ho PS]] |
| - | ==About this Structure== | + | [[Category: Jones ZJR]] |
| - | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZFH OCA]. | + | [[Category: Raup D]] |
| - | | + | [[Category: Teegarden AT]] |
| - | ==Reference== | + | [[Category: Watson J]] |
| - | How sequence defines structure: a crystallographic map of DNA structure and conformation., Hays FA, Teegarden A, Jones ZJ, Harms M, Raup D, Watson J, Cavaliere E, Ho PS, Proc Natl Acad Sci U S A. 2005 May 17;102(20):7157-62. Epub 2005 May 3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15870206 15870206]
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| - | [[Category: Cavaliere, E.]] | + | |
| - | [[Category: Harms, M.]] | + | |
| - | [[Category: Hays, F A.]] | + | |
| - | [[Category: Ho, P S.]] | + | |
| - | [[Category: Jones, Z J.R.]] | + | |
| - | [[Category: Raup, D.]] | + | |
| - | [[Category: Teegarden, A T.]] | + | |
| - | [[Category: Watson, J.]] | + | |
| - | [[Category: Crystallographic screen]] | + | |
| - | [[Category: Dna structure]] | + | |
| - | [[Category: Holliday junction]] | + | |
| - | [[Category: Molecular structure]] | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:33:39 2008''
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