1zfn

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[[Image:1zfn.gif|left|200px]]
 
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==Structural Analysis of Escherichia coli ThiF==
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The line below this paragraph, containing "STRUCTURE_1zfn", creates the "Structure Box" on the page.
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<StructureSection load='1zfn' size='340' side='right'caption='[[1zfn]], [[Resolution|resolution]] 2.75&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1zfn]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZFN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZFN FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.75&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_1zfn| PDB=1zfn | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zfn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zfn OCA], [https://pdbe.org/1zfn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zfn RCSB], [https://www.ebi.ac.uk/pdbsum/1zfn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zfn ProSAT]</span></td></tr>
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</table>
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'''Structural Analysis of Escherichia coli ThiF'''
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== Function ==
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[https://www.uniprot.org/uniprot/THIF_ECOLI THIF_ECOLI] Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS.
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Escherichia coli ThiF is an enzyme in the biosynthetic cascade for generating the essential cofactor thiamin pyrophosphate. In this cascade, ThiF catalyzes adenylation of the C terminus of ThiS. We report here the crystal structures of ThiF, alone and in complex with ATP. The structures provide insight into a preference for ATP during adenylation of the protein ThiS. Additionally, the structures reveal an ordered crossover loop predicted to clamp the flexible tail of ThiS into the ThiF active site during the adenylation reaction. The importance of the crossover loop for ThiF activity is highlighted by mutational analysis. Comparison of ThiF with the structural homologues MoeB, APPBP1-UBA3, and SAE1-SAE2 reveals that the ATP-binding site, including an arginine-finger, is maintained throughout evolution, and shows divergence occurring in protein substrate-binding sites and regions devoted to unique steps in the specific function of each enzyme.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zf/1zfn_consurf.spt"</scriptWhenChecked>
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1ZFN is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZFN OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Structural analysis of Escherichia coli ThiF., Duda DM, Walden H, Sfondouris J, Schulman BA, J Mol Biol. 2005 Jun 17;349(4):774-86. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15896804 15896804]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zfn ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Duda, D M.]]
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[[Category: Duda DM]]
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[[Category: Schulman, B A.]]
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[[Category: Schulman BA]]
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[[Category: Sfondouris, J.]]
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[[Category: Sfondouris J]]
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[[Category: Walden, H.]]
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[[Category: Walden H]]
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[[Category: Adenylation]]
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[[Category: Atp-binding]]
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[[Category: P-loop]]
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[[Category: Rossman fold]]
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[[Category: Thif]]
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[[Category: These]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:34:03 2008''
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Current revision

Structural Analysis of Escherichia coli ThiF

PDB ID 1zfn

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