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1zhi

From Proteopedia

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[[Image:1zhi.gif|left|200px]]
 
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==Complex of the S. cerevisiae Orc1 and Sir1 interacting domains==
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The line below this paragraph, containing "STRUCTURE_1zhi", creates the "Structure Box" on the page.
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<StructureSection load='1zhi' size='340' side='right'caption='[[1zhi]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1zhi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZHI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZHI FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zhi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zhi OCA], [https://pdbe.org/1zhi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zhi RCSB], [https://www.ebi.ac.uk/pdbsum/1zhi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zhi ProSAT]</span></td></tr>
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{{STRUCTURE_1zhi| PDB=1zhi | SCENE= }}
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</table>
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== Function ==
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'''Complex of the S. cerevisiae Orc1 and Sir1 interacting domains'''
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[https://www.uniprot.org/uniprot/ORC1_YEAST ORC1_YEAST] Component of the origin recognition complex (ORC) that binds origins of replication. It has a role in both chromosomal replication and mating type transcriptional silencing. Binds to the ARS consensus sequence (ACS) of origins of replication.<ref>PMID:17825064</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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The Sir1 protein plays a key role in establishing a silent chromatin structure at the cryptic mating-type loci HMR and HML in Saccharomyces cerevisiae by interacting with the bromo-adjacent homology (BAH) domain of the Orc1p subunit of the origin recognition complex (ORC). Here, we present the high-resolution crystal structures of the ORC interaction region (OIR) of Sir1p and that of the complex formed between the OIR and BAH domains. Amino acids within the OIR previously shown to be required for a Sir1p/ORC interaction are presented on a conserved, convex surface that forms a complementary interface with a concave region of the Orc1 BAH domain that is critical for transcriptional silencing. The OIR/BAH interaction surface comprises a network of hydrophobic and polar/ionic interactions between discrete structural modules in each protein and involves several residues that were not implicated in previous studies. These data provide important structural insights into a protein-protein interaction critical for the formation of a specialized chromatin domain within eukaryotic chromosomes.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zh/1zhi_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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1ZHI is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZHI OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zhi ConSurf].
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Structural basis of the Sir1-origin recognition complex interaction in transcriptional silencing., Hou Z, Bernstein DA, Fox CA, Keck JL, Proc Natl Acad Sci U S A. 2005 Jun 14;102(24):8489-94. Epub 2005 Jun 2. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15932939 15932939]
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<div style="clear:both"></div>
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[[Category: Protein complex]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Bernstein, D A.]]
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[[Category: Bernstein DA]]
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[[Category: Fox, C A.]]
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[[Category: Fox CA]]
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[[Category: Hou, Z.]]
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[[Category: Hou Z]]
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[[Category: Keck, J L.]]
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[[Category: Keck JL]]
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[[Category: Bah domain]]
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[[Category: Oir domain]]
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[[Category: Protein complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:37:47 2008''
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Current revision

Complex of the S. cerevisiae Orc1 and Sir1 interacting domains

PDB ID 1zhi

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