2cy7

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(New page: 200px<br /> <applet load="2cy7" size="450" color="white" frame="true" align="right" spinBox="true" caption="2cy7, resolution 1.90&Aring;" /> '''The crystal structu...)
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[[Image:2cy7.gif|left|200px]]<br />
 
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<applet load="2cy7" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2cy7, resolution 1.90&Aring;" />
 
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'''The crystal structure of human Atg4B'''<br />
 
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==Overview==
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==The crystal structure of human Atg4B==
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Reversible modification of Atg8 with phosphatidylethanolamine is crucial, for autophagy, the bulk degradation system conserved in eukaryotic cells., Atg4 is a novel cysteine protease that processes and deconjugates Atg8., Herein, we report the crystal structure of human Atg4B (HsAtg4B) at 1.9-A, resolution. Despite no obvious sequence homology with known proteases, the, structure of HsAtg4B shows a classical papain-like fold. In addition to, the papain fold region, HsAtg4B has a small alpha/beta-fold domain. This, domain is thought to be the binding site for Atg8 homologs. The active, site cleft of HsAtg4B is masked by a loop (residues 259-262), implying a, conformational change upon substrate binding. The structure and in vitro, mutational analyses provide the basis for the specificity and catalysis of, HsAtg4B. This will enable the design of Atg4-specific inhibitors that, block autophagy.
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<StructureSection load='2cy7' size='340' side='right'caption='[[2cy7]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2cy7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CY7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CY7 FirstGlance]. <br>
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2CY7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CY7 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cy7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cy7 OCA], [https://pdbe.org/2cy7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cy7 RCSB], [https://www.ebi.ac.uk/pdbsum/2cy7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cy7 ProSAT]</span></td></tr>
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==Reference==
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</table>
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Structural basis for the specificity and catalysis of human Atg4B responsible for mammalian autophagy., Sugawara K, Suzuki NN, Fujioka Y, Mizushima N, Ohsumi Y, Inagaki F, J Biol Chem. 2005 Dec 2;280(48):40058-65. Epub 2005 Sep 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16183633 16183633]
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== Function ==
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[https://www.uniprot.org/uniprot/ATG4B_HUMAN ATG4B_HUMAN] Cysteine protease required for autophagy, which cleaves the C-terminal part of MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Also mediates the lipid deconjugation required for target recycling.<ref>PMID:15169837</ref> <ref>PMID:19322194</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cy/2cy7_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cy7 ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Fujioka, Y.]]
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[[Category: Fujioka Y]]
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[[Category: Inagaki, F.]]
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[[Category: Inagaki F]]
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[[Category: Mizushima, N.]]
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[[Category: Mizushima N]]
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[[Category: Ohsumi, Y.]]
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[[Category: Ohsumi Y]]
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[[Category: Sugawara, K.]]
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[[Category: Sugawara K]]
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[[Category: Suzuki, N.N.]]
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[[Category: Suzuki NN]]
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[[Category: autophagy]]
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[[Category: papain-like fold]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 21:24:34 2007''
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Current revision

The crystal structure of human Atg4B

PDB ID 2cy7

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