1zuw

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[[Image:1zuw.gif|left|200px]]
 
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==Crystal structure of B.subtilis glutamate racemase (RacE) with D-Glu==
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The line below this paragraph, containing "STRUCTURE_1zuw", creates the "Structure Box" on the page.
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<StructureSection load='1zuw' size='340' side='right'caption='[[1zuw]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1zuw]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZUW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZUW FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DGL:D-GLUTAMIC+ACID'>DGL</scene></td></tr>
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{{STRUCTURE_1zuw| PDB=1zuw | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zuw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zuw OCA], [https://pdbe.org/1zuw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zuw RCSB], [https://www.ebi.ac.uk/pdbsum/1zuw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zuw ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MURI1_BACSU MURI1_BACSU] Provides the (R)-glutamate required for cell wall biosynthesis (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zu/1zuw_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zuw ConSurf].
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<div style="clear:both"></div>
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'''Crystal structure of B.subtilis glutamate racemase (RacE) with D-Glu'''
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==See Also==
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*[[Glutamate racemase 3D structures|Glutamate racemase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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D-glutamate is an essential building block of the peptidoglycan layer in bacterial cell walls and can be synthesized from L-glutamate by glutamate racemase (RacE). The structure of a complex of B. subtilis RacE with D-glutamate reveals that the glutamate is buried in a deep pocket, whose formation at the interface of the enzyme's two domains involves a large-scale conformational rearrangement. These domains are related by pseudo-2-fold symmetry, which superimposes the two catalytic cysteine residues, which are located at equivalent positions on either side of the alpha carbon of the substrate. The structural similarity of these two domains suggests that the racemase activity of RacE arose as a result of gene duplication. The structure of the complex is dramatically different from that proposed previously and provides new insights into the RacE mechanism and an explanation for the potency of a family of RacE inhibitors, which have been developed as novel antibiotics.
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==About this Structure==
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1ZUW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZUW OCA].
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==Reference==
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Substrate-induced conformational changes in Bacillus subtilis glutamate racemase and their implications for drug discovery., Ruzheinikov SN, Taal MA, Sedelnikova SE, Baker PJ, Rice DW, Structure. 2005 Nov;13(11):1707-13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16271894 16271894]
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[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
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[[Category: Glutamate racemase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Baker PJ]]
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[[Category: Baker, P J.]]
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[[Category: Rice DW]]
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[[Category: Rice, D W.]]
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[[Category: Ruzheinikov SN]]
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[[Category: Ruzheinikov, S N.]]
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[[Category: Sedelnikova SE]]
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[[Category: Sedelnikova, S E.]]
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[[Category: Taal MA]]
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[[Category: Taal, M A.]]
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[[Category: Glutamate racemase]]
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[[Category: Peptidoglycan biosynthesis]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:06:11 2008''
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Current revision

Crystal structure of B.subtilis glutamate racemase (RacE) with D-Glu

PDB ID 1zuw

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