1zwx

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[[Image:1zwx.gif|left|200px]]
 
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==Crystal Structure of SmcL==
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The line below this paragraph, containing "STRUCTURE_1zwx", creates the "Structure Box" on the page.
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<StructureSection load='1zwx' size='340' side='right'caption='[[1zwx]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1zwx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Listeria_ivanovii Listeria ivanovii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZWX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZWX FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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{{STRUCTURE_1zwx| PDB=1zwx | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zwx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zwx OCA], [https://pdbe.org/1zwx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zwx RCSB], [https://www.ebi.ac.uk/pdbsum/1zwx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zwx ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PHLC_LISIV PHLC_LISIV]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zw/1zwx_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zwx ConSurf].
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<div style="clear:both"></div>
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'''Crystal Structure of SmcL'''
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==See Also==
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*[[Sphingomyelinase|Sphingomyelinase]]
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__TOC__
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==Overview==
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</StructureSection>
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Sphingomyelinases C are enzymes that catalyze the hydrolysis of sphingomyelin in biological membranes to ceramide and phosphorylcholine. Various pathogenic bacteria produce secreted neutral sphingomyelinases C that act as membrane-damaging virulence factors. Mammalian neutral sphingomyelinases C, which display sequence homology to the bacterial enzymes, are involved in sphingolipid metabolism and signaling. This article describes the first structure to be determined for a member of the neutral sphingomyelinase C family, SmcL, from the intracellular bacterial pathogen Listeria ivanovii. The structure has been refined to 1.9-A resolution with phases derived by single isomorphous replacement with anomalous scattering techniques from a single iridium derivative. SmcL adopts a DNase I-like fold, and is the first member of this protein superfamily to have its structure determined that acts as a phospholipase. The structure reveals several unique features that adapt the protein to its phospholipid substrate. These include large hydrophobic beta-hairpin and hydrophobic loops surrounding the active site that may bind and penetrate the lipid bilayer to position sphingomyelin in a catalytically competent position. The structure also provides insight into the proposed general base/acid catalytic mechanism, in which His-325 and His-185 play key roles.
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[[Category: Large Structures]]
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==About this Structure==
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1ZWX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Listeria_ivanovii Listeria ivanovii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZWX OCA].
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==Reference==
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Crystal structure of SmcL, a bacterial neutral sphingomyelinase C from Listeria., Openshaw AE, Race PR, Monzo HJ, Vazquez-Boland JA, Banfield MJ, J Biol Chem. 2005 Oct 14;280(41):35011-7. Epub 2005 Aug 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16093240 16093240]
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[[Category: Listeria ivanovii]]
[[Category: Listeria ivanovii]]
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[[Category: Single protein]]
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[[Category: Banfield MJ]]
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[[Category: Sphingomyelin phosphodiesterase]]
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[[Category: Monzo HJ]]
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[[Category: Banfield, M J.]]
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[[Category: Openshaw AEA]]
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[[Category: Monzo, H J.]]
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[[Category: Race PR]]
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[[Category: Openshaw, A E.A.]]
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[[Category: Vasquez-Boland JA]]
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[[Category: Race, P R.]]
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[[Category: Vasquez-Boland, J A.]]
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[[Category: Beta-hairpin]]
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[[Category: Dnase1-like fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:10:43 2008''
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Current revision

Crystal Structure of SmcL

PDB ID 1zwx

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