1zwz

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[[Image:1zwz.gif|left|200px]]
 
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==Structural comparison of Yeast snoRNP and splicesomal protein snu13p with its homologs==
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The line below this paragraph, containing "STRUCTURE_1zwz", creates the "Structure Box" on the page.
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<StructureSection load='1zwz' size='340' side='right'caption='[[1zwz]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1zwz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZWZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZWZ FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zwz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zwz OCA], [https://pdbe.org/1zwz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zwz RCSB], [https://www.ebi.ac.uk/pdbsum/1zwz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zwz ProSAT]</span></td></tr>
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{{STRUCTURE_1zwz| PDB=1zwz | SCENE= }}
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</table>
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== Function ==
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'''Structural comparison of Yeast snoRNP and splicesomal protein snu13p with its homologs'''
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[https://www.uniprot.org/uniprot/SNU13_YEAST SNU13_YEAST] Common component of the spliceosome and rRNA processing machinery. In association with the spliceosomal U4/U6.U5 tri-snRNP particle, required for splicing of pre-mRNA. In association with box C/D snoRNPs, required for processing of pre-ribosomal RNA (rRNA) and site-specific 2'-O-methylation of substrate RNAs. Essential for the accumulation and stability of U4 snRNA, U6 snRNA, and box C/D snoRNAs.<ref>PMID:11081632</ref> <ref>PMID:12215523</ref> <ref>PMID:14730029</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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Snu13p is a bifunctional yeast protein involved in both messenger RNA splicing as well as ribosomal RNA maturation. Snu13p initiates assembly of ribonucleoprotein particles by interacting with a conserved RNA motif called kink turn. Unlike its archaeal homolog, L7Ae, Snu13p displays differential specificity for functionally distinct kink turns. Thus, the structures of Snu13p at different functional states, including those alone and bound with RNAs, are required to understand how the protein differentially interacts with kink turns. Although the structure of the human homolog of Snu13p bound with a spliceosomal RNA is known, there has not been a report of a structure of free Snu13p. This has hindered our ability to understand the structural basis for Snu13p's substrate specificity. We report a crystal structure of free Snu13p at 1.9A and a detailed structural comparison with its homologs. We show that free Snu13p has nearly an identical conformation as that of its human homolog bound with RNA. Interestingly, both eukaryotic proteins exhibit notable structural differences in their central beta-sheets as compared to their archaeal homolog, L7Ae. The observed structural differences offer a possible explanation to the observed difference in RNA specificity between Snu13p and L7Ae.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zw/1zwz_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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1ZWZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZWZ OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zwz ConSurf].
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Structural comparison of yeast snoRNP and spliceosomal protein Snu13p with its homologs., Oruganti S, Zhang Y, Li H, Biochem Biophys Res Commun. 2005 Jul 29;333(2):550-4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15963469 15963469]
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Li H]]
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[[Category: Li, H.]]
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[[Category: Oruganti S]]
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[[Category: Oruganti, S.]]
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[[Category: Zhang Y]]
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[[Category: Zhang, Y.]]
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[[Category: Mrna splicing]]
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[[Category: Protein rna complex]]
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[[Category: Rrna modification]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:10:50 2008''
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Current revision

Structural comparison of Yeast snoRNP and splicesomal protein snu13p with its homologs

PDB ID 1zwz

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