227d

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[[Image:227d.jpg|left|200px]]
 
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==A CRYSTALLOGRAPHIC AND SPECTROSCOPIC STUDY OF THE COMPLEX BETWEEN D(CGCGAATTCGCG)2 AND 2,5-BIS(4-GUANYLPHENYL)FURAN, AN ANALOGUE OF BERENIL. STRUCTURAL ORIGINS OF ENHANCED DNA-BINDING AFFINITY==
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The line below this paragraph, containing "STRUCTURE_227d", creates the "Structure Box" on the page.
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<StructureSection load='227d' size='340' side='right'caption='[[227d]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[227d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=227D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=227D FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGF:2,5-BIS(4-GUANYLPHENYL)FURAN'>BGF</scene></td></tr>
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{{STRUCTURE_227d| PDB=227d | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=227d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=227d OCA], [https://pdbe.org/227d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=227d RCSB], [https://www.ebi.ac.uk/pdbsum/227d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=227d ProSAT]</span></td></tr>
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</table>
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'''A CRYSTALLOGRAPHIC AND SPECTROSCOPIC STUDY OF THE COMPLEX BETWEEN D(CGCGAATTCGCG)2 AND 2,5-BIS(4-GUANYLPHENYL)FURAN, AN ANALOGUE OF BERENIL. STRUCTURAL ORIGINS OF ENHANCED DNA-BINDING AFFINITY'''
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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==Overview==
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[[Category: Boykin DW]]
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2,5-Bis(4-guanylphenyl)furan ("furamidine") is a dicationic minor groove binding drug that has been shown to be more effective than pentamidine against the Pneumocystis carinii pathogen in an immunosuppressed rat model. It has a close structural similarity to the antitrypanosomal drug berenil, differing only on the replacement of the central triazene unit with a furan moiety. we have determined the crystal structure of the complex between furamidine and the DNA dodecamer d(CGCGAATTCGCG)2 and compared it with the same DNA sequence by UV-visible, fluorescence, and CD spectroscopy. Furamidine shows tighter binding to this sequence (Keq = 6.7 x 10(6)) than berenil (Keq = 6.6 x 10(5)). The crystal structure reveals that, unlike berenil, furamidine makes direct hydrogen bond interactions with this DNA sequence through both amidinium groups to O2 atoms of thymine bases and is more isohelical with the minor groove. Molecular mechanics calculations support the hypothesis that these differences result in the improved interaction energy between the ligand and the DNA.
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[[Category: Laughton CA]]
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[[Category: Neidle S]]
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==About this Structure==
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[[Category: Nunn CM]]
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=227D OCA].
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[[Category: Tanious F]]
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[[Category: Wilson WD]]
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==Reference==
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A crystallographic and spectroscopic study of the complex between d(CGCGAATTCGCG)2 and 2,5-bis(4-guanylphenyl)furan, an analogue of berenil. Structural origins of enhanced DNA-binding affinity., Laughton CA, Tanious F, Nunn CM, Boykin DW, Wilson WD, Neidle S, Biochemistry. 1996 May 7;35(18):5655-61. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8639524 8639524]
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[[Category: Boykin, D W.]]
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[[Category: Laughton, C A.]]
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[[Category: Neidle, S.]]
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[[Category: Nunn, C M.]]
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[[Category: Tanious, F.]]
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[[Category: Wilson, W D.]]
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[[Category: B-dna]]
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[[Category: Complexed with drug]]
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[[Category: Double helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:20:22 2008''
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Current revision

A CRYSTALLOGRAPHIC AND SPECTROSCOPIC STUDY OF THE COMPLEX BETWEEN D(CGCGAATTCGCG)2 AND 2,5-BIS(4-GUANYLPHENYL)FURAN, AN ANALOGUE OF BERENIL. STRUCTURAL ORIGINS OF ENHANCED DNA-BINDING AFFINITY

PDB ID 227d

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