230d

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[[Image:230d.gif|left|200px]]
 
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==SOLUTION STRUCTURES OF UNIMOLECULAR QUADRUPLEXES FORMED BY OLIGONUCLEOTIDES CONTAINING OXYTRICHA TELOMERE REPEATS==
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The line below this paragraph, containing "STRUCTURE_230d", creates the "Structure Box" on the page.
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<StructureSection load='230d' size='340' side='right'caption='[[230d]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[230d]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=230D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=230D FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=230d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=230d OCA], [https://pdbe.org/230d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=230d RCSB], [https://www.ebi.ac.uk/pdbsum/230d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=230d ProSAT]</span></td></tr>
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{{STRUCTURE_230d| PDB=230d | SCENE= }}
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</table>
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<div style="background-color:#fffaf0;">
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'''SOLUTION STRUCTURES OF UNIMOLECULAR QUADRUPLEXES FORMED BY OLIGONUCLEOTIDES CONTAINING OXYTRICHA TELOMERE REPEATS'''
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== Publication Abstract from PubMed ==
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==Overview==
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BACKGROUND: Oligonucleotides containing the guanine-rich telomeric sequence of Oxytricha chromosomes (dT4G4) have previously been shown to form DNA quadruplexes comprising guanine quartets stabilized by cations. Two different structures have been reported for both d(G4T4G4) (Oxy1.5) and d(G4T4G4T4G4T4G4) (Oxy3.5). RESULTS: Here we present the solution structure of a uracil- and inosine-containing derivative of Oxy3.5, d(G4TUTUG4T4G4UUTTG3I) (Oxy3.5-U4128), determined using two-dimensional 1H and 31P NMR techniques. This oligonucleotide forms a unimolecular quadruplex that is very similar to the dimeric Oxy1.5 solution structure, in that it contains a loop spanning the diagonal of an end quartet. The groove widths, strand polarities, and positions of the syn bases along the G4 tracts and within the quartets are all as reported for Oxy1.5. The first and third pyrimidine tracts form parallel loops spanning a wide groove and a narrow groove respectively. CONCLUSIONS: Both Oxy3.5 and Oxy3.5-U(4)128 form unimolecular quadruplexes in solution with a diagonal central T4 loop. These results conflict with those reported for d(G4TUTUG4TTUUG4UUTTG4) in solution, in which the central loop spans a wide groove.
BACKGROUND: Oligonucleotides containing the guanine-rich telomeric sequence of Oxytricha chromosomes (dT4G4) have previously been shown to form DNA quadruplexes comprising guanine quartets stabilized by cations. Two different structures have been reported for both d(G4T4G4) (Oxy1.5) and d(G4T4G4T4G4T4G4) (Oxy3.5). RESULTS: Here we present the solution structure of a uracil- and inosine-containing derivative of Oxy3.5, d(G4TUTUG4T4G4UUTTG3I) (Oxy3.5-U4128), determined using two-dimensional 1H and 31P NMR techniques. This oligonucleotide forms a unimolecular quadruplex that is very similar to the dimeric Oxy1.5 solution structure, in that it contains a loop spanning the diagonal of an end quartet. The groove widths, strand polarities, and positions of the syn bases along the G4 tracts and within the quartets are all as reported for Oxy1.5. The first and third pyrimidine tracts form parallel loops spanning a wide groove and a narrow groove respectively. CONCLUSIONS: Both Oxy3.5 and Oxy3.5-U(4)128 form unimolecular quadruplexes in solution with a diagonal central T4 loop. These results conflict with those reported for d(G4TUTUG4TTUUG4UUTTG4) in solution, in which the central loop spans a wide groove.
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==About this Structure==
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Solution structures of unimolecular quadruplexes formed by oligonucleotides containing Oxytricha telomere repeats.,Smith FW, Schultze P, Feigon J Structure. 1995 Oct 15;3(10):997-1008. PMID:8590010<ref>PMID:8590010</ref>
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=230D OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Solution structures of unimolecular quadruplexes formed by oligonucleotides containing Oxytricha telomere repeats., Smith FW, Schultze P, Feigon J, Structure. 1995 Oct 15;3(10):997-1008. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8590010 8590010]
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</div>
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[[Category: Feigon, J.]]
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<div class="pdbe-citations 230d" style="background-color:#fffaf0;"></div>
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[[Category: Schultze, P.]]
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== References ==
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[[Category: Smith, F W.]]
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<references/>
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[[Category: Dna]]
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__TOC__
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[[Category: Nmr]]
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</StructureSection>
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[[Category: Oxytricha telomere repeat]]
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[[Category: Large Structures]]
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[[Category: Quadruplex]]
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[[Category: Feigon J]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:20:50 2008''
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[[Category: Schultze P]]
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[[Category: Smith FW]]

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SOLUTION STRUCTURES OF UNIMOLECULAR QUADRUPLEXES FORMED BY OLIGONUCLEOTIDES CONTAINING OXYTRICHA TELOMERE REPEATS

PDB ID 230d

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