233d

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[[Image:233d.gif|left|200px]]
 
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==THE CRYSTAL STRUCTURE ANALYSIS OF D(CGCGAASSCGCG)2: A SYNTHETIC DNA DODECAMER DUPLEX CONTAINING FOUR 4'-THIO-2'-DEOXYTHYMIDINE NUCLEOTIDES==
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The line below this paragraph, containing "STRUCTURE_233d", creates the "Structure Box" on the page.
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<StructureSection load='233d' size='340' side='right'caption='[[233d]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[233d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=233D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=233D FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=T49:S4-2DEOXYTHYMIDINE+5-MONOPHOSPHATE'>T49</scene></td></tr>
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{{STRUCTURE_233d| PDB=233d | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=233d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=233d OCA], [https://pdbe.org/233d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=233d RCSB], [https://www.ebi.ac.uk/pdbsum/233d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=233d ProSAT]</span></td></tr>
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</table>
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'''THE CRYSTAL STRUCTURE ANALYSIS OF D(CGCGAASSCGCG)2: A SYNTHETIC DNA DODECAMER DUPLEX CONTAINING FOUR 4'-THIO-2'-DEOXYTHYMIDINE NUCLEOTIDES'''
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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==Overview==
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[[Category: Boggon TJ]]
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The crystal structure refinement of the synthetic dodecamer d(CGCGAASSCGCG), where S = 4'-thio-2'-deoxythymidine, has converged at R=0.201 for 2605 reflections with F &gt; 2sigma(F) in the resolution range 8.0-2.4 A for a model consisting of the dodecamer duplex and 66 water molecules. A comparison of its structure with that of the native dodecamer d(CGCGAATTCGCG) has revealed that the major differences between the two structures is a change in the conformation of the sugar-phosphate backbone in the regions at and adjacent to the positions of the modified nucleosides. Examination of the fine structural parameters for each of the structures reveals that the thiosugars adopt a C3'-exo conformation in d(CGCGAASSCGCG), rather than the approximate C1'-exo conformation found for the analogous sugars in the structure of d(CGCGAATTCGCG). The observed differences in structure between the two duplexes may help to explain the enhanced resistance to nuclease digestion of synthetic oligonucleotides containing 4'-thio-2'-deoxynucleotides.
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[[Category: Brown T]]
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[[Category: Connolly BA]]
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==About this Structure==
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[[Category: Hancox EL]]
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=233D OCA].
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[[Category: Hunter WN]]
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[[Category: Leonard GA]]
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==Reference==
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[[Category: McAuley-Hecht KE]]
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The crystal structure analysis of d(CGCGAASSCGCG)2, a synthetic DNA dodecamer duplex containing four 4'-thio-2'-deoxythymidine nucleotides., Boggon TJ, Hancox EL, McAuley-Hecht KE, Connolly BA, Hunter WN, Brown T, Walker RT, Leonard GA, Nucleic Acids Res. 1996 Mar 1;24(5):951-61. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8600465 8600465]
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[[Category: Walker RT]]
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[[Category: Boggon, T J.]]
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[[Category: Brown, T.]]
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[[Category: Connolly, B A.]]
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[[Category: Hancox, E L.]]
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[[Category: Hunter, W N.]]
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[[Category: Leonard, G A.]]
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[[Category: McAuley-Hecht, K E.]]
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[[Category: Walker, R T.]]
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[[Category: B-dna]]
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[[Category: Double helix]]
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[[Category: Modified]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:21:13 2008''
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Current revision

THE CRYSTAL STRUCTURE ANALYSIS OF D(CGCGAASSCGCG)2: A SYNTHETIC DNA DODECAMER DUPLEX CONTAINING FOUR 4'-THIO-2'-DEOXYTHYMIDINE NUCLEOTIDES

PDB ID 233d

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