296d

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[[Image:296d.gif|left|200px]]
 
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==SEQUENCE-DEPENDENT EFFECTS IN DRUG-DNA INTERACTION: THE CRYSTAL STRUCTURE OF HOECHST 33258 BOUND TO THE D(CGCAAATTTGCG)2 DUPLEX==
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The line below this paragraph, containing "STRUCTURE_296d", creates the "Structure Box" on the page.
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<StructureSection load='296d' size='340' side='right'caption='[[296d]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[296d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=296D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=296D FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HT:2-(4-HYDROXYPHENYL)-5-(4-METHYL-1-PIPERAZINYL)-2,5-BI-BENZIMIDAZOLE'>HT</scene></td></tr>
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{{STRUCTURE_296d| PDB=296d | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=296d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=296d OCA], [https://pdbe.org/296d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=296d RCSB], [https://www.ebi.ac.uk/pdbsum/296d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=296d ProSAT]</span></td></tr>
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</table>
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'''SEQUENCE-DEPENDENT EFFECTS IN DRUG-DNA INTERACTION: THE CRYSTAL STRUCTURE OF HOECHST 33258 BOUND TO THE D(CGCAAATTTGCG)2 DUPLEX'''
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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==Overview==
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[[Category: Brown DG]]
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The bis-benzimidazole drug Hoechst 33258 has been co-crystallized with the dodecanucleotide sequence d(CGCAAATTTGCG)2. The structure has been solved by molecular replacement and refined to an R factor of 18.5% for 2125 reflections collected on a Xentronics area detector. The drug is bound in the minor groove, at the five base-pair site 5'-ATTTG and is in a unique orientation. This is displaced by one base pair in the 5' direction compared to previously-determined structures of this drug with the sequence d(CGCGAATTCGCG)2. Reasons for this difference in behaviour are discussed in terms of several sequence-dependent structural features of the DNA, with particular reference to differences in propeller twist and minor-groove width.
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[[Category: Neidle S]]
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[[Category: Skelly JV]]
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==About this Structure==
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[[Category: Spink N]]
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=296D OCA].
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==Reference==
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Sequence-dependent effects in drug-DNA interaction: the crystal structure of Hoechst 33258 bound to the d(CGCAAATTTGCG)2 duplex., Spink N, Brown DG, Skelly JV, Neidle S, Nucleic Acids Res. 1994 May 11;22(9):1607-12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7515488 7515488]
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[[Category: Brown, D G.]]
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[[Category: Neidle, S.]]
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[[Category: Skelly, J V.]]
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[[Category: Spink, N.]]
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[[Category: B-dna]]
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[[Category: Complexed with drug]]
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[[Category: Double helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:26:08 2008''
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Current revision

SEQUENCE-DEPENDENT EFFECTS IN DRUG-DNA INTERACTION: THE CRYSTAL STRUCTURE OF HOECHST 33258 BOUND TO THE D(CGCAAATTTGCG)2 DUPLEX

PDB ID 296d

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