2a4x

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[[Image:2a4x.gif|left|200px]]
 
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==Crystal Structure Of Mitomycin C-Binding Protein Complexed with Metal-Free Bleomycin A2==
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The line below this paragraph, containing "STRUCTURE_2a4x", creates the "Structure Box" on the page.
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<StructureSection load='2a4x' size='340' side='right'caption='[[2a4x]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2a4x]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_caespitosus Streptomyces caespitosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A4X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A4X FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BLM:BLEOMYCIN+A2'>BLM</scene></td></tr>
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{{STRUCTURE_2a4x| PDB=2a4x | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a4x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a4x OCA], [https://pdbe.org/2a4x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a4x RCSB], [https://www.ebi.ac.uk/pdbsum/2a4x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a4x ProSAT]</span></td></tr>
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</table>
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'''Crystal Structure Of Mitomycin C-Binding Protein Complexed with Metal-Free Bleomycin A2'''
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== Function ==
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[https://www.uniprot.org/uniprot/O05205_STRLA O05205_STRLA]
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Antibiotic-producing microorganisms must be protected from the lethal effect of their own antibiotic. We have previously determined the X-ray crystal structure of the bleomycin (Bm)-binding protein, designated BLMA, as a self-resistance determinant from Bm-producing Streptomyces verticillus, which suggests that the binding of the first Bm to one of two pockets formed in the BLMA homodimer induces the cooperative binding of the second Bm to the other pocket. In the present study, we noticed that the X-ray crystallographic structure of a self-resistance determinant from a mitomycin C-producing microorganism, designated MRDP, reveals similarity to the folding pattern on the BLMA, although no sequence homology exists. To clarify the hypothesis that MRDP may function as a resistance determinant to Bm, we characterized and determined the crystal structure of MRDP complexed with the Cu(II)-bound form of BmA(2) grouped into the Bm family of antibiotics. The biochemical and structural studies for Bm binding provide evidence that the first Bm binds anti-cooperatively to a pocket of MRDP with binding affinity of the nanomolar order, whereas the second Bm binds to the other pocket, which has binding affinity of the micromolar order. The invisibility of the second Bm in the structure agrees with the observation that Escherichia coli-expressing MRDP displays lower resistance to Bm than that expressing BLMA. The structure of MRDP, which is complexed with the Cu(II)-bound BmA(2), revealed that the gamma-aminopropyldimethylsulphonium moiety of the antibiotic is sandwiched between the peripheral residues of the binding pocket and that its positively charged sulphonium head is accommodated completely in the negatively charged region of the MRDP pocket. Furthermore, the Cu(II)-bound BmA(2) has a very compact structure, in which the bithiazole ring of BmA(2) is folded back to the metal-binding domain.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a4/2a4x_consurf.spt"</scriptWhenChecked>
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2A4X is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_caespitosus Streptomyces caespitosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A4X OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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The mitomycin C (MMC)-binding protein from MMC-producing microorganisms protects from the lethal effect of bleomycin: crystallographic analysis to elucidate the binding mode of the antibiotic to the protein., Danshiitsoodol N, de Pinho CA, Matoba Y, Kumagai T, Sugiyama M, J Mol Biol. 2006 Jul 7;360(2):398-408. Epub 2006 May 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16756991 16756991]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2a4x ConSurf].
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[[Category: Single protein]]
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Streptomyces caespitosus]]
[[Category: Streptomyces caespitosus]]
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[[Category: Danshiitsoodol, N.]]
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[[Category: Danshiitsoodol N]]
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[[Category: Kumagai, T.]]
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[[Category: Kumagai T]]
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[[Category: Matoba, Y.]]
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[[Category: Matoba Y]]
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[[Category: Pinho, C A.de.]]
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[[Category: Sugiyama M]]
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[[Category: Sugiyama, M.]]
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[[Category: De Pinho CA]]
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[[Category: Alfa/beta protein]]
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[[Category: Bleomycin a2]]
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[[Category: Mitomycin c-binding protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:36:23 2008''
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Current revision

Crystal Structure Of Mitomycin C-Binding Protein Complexed with Metal-Free Bleomycin A2

PDB ID 2a4x

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