2afp

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[[Image:2afp.jpg|left|200px]]
 
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==THE SOLUTION STRUCTURE OF TYPE II ANTIFREEZE PROTEIN REVEALS A NEW MEMBER OF THE LECTIN FAMILY==
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The line below this paragraph, containing "STRUCTURE_2afp", creates the "Structure Box" on the page.
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<StructureSection load='2afp' size='340' side='right'caption='[[2afp]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2afp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Hemitripterus_americanus Hemitripterus americanus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AFP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AFP FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 5 models</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2afp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2afp OCA], [https://pdbe.org/2afp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2afp RCSB], [https://www.ebi.ac.uk/pdbsum/2afp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2afp ProSAT]</span></td></tr>
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{{STRUCTURE_2afp| PDB=2afp | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ISP2_HEMAM ISP2_HEMAM] Antifreeze proteins lower the blood freezing point.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/af/2afp_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2afp ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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A recombinant form of the sea raven type II antifreeze protein (SRAFP) has been produced using the Pichia pastoris expression system. The antifreeze activity of recombinant SRAFP is indistinguishable from that of the wild-type protein. The global fold of SRAFP has been determined by two-dimensional 1H homonuclear and three-dimensional 1H- inverted question mark15N inverted question mark heteronuclear NMR spectroscopy using 785 NOE distance restraints and 47 angular restraints. The molecule folds into one globular domain that consists of two helices and nine beta-strands in two beta-sheets. The structure confirms the proposed existence of five disulfide bonds. The global fold of SRAFP is homologous to C-type lectins and pancreatic stone proteins, even though the sequence identity is only approximately 20%.
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'''THE SOLUTION STRUCTURE OF TYPE II ANTIFREEZE PROTEIN REVEALS A NEW MEMBER OF THE LECTIN FAMILY'''
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The solution structure of type II antifreeze protein reveals a new member of the lectin family.,Gronwald W, Loewen MC, Lix B, Daugulis AJ, Sonnichsen FD, Davies PL, Sykes BD Biochemistry. 1998 Apr 7;37(14):4712-21. PMID:9537986<ref>PMID:9537986</ref>
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==Overview==
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A recombinant form of the sea raven type II antifreeze protein (SRAFP) has been produced using the Pichia pastoris expression system. The antifreeze activity of recombinant SRAFP is indistinguishable from that of the wild-type protein. The global fold of SRAFP has been determined by two-dimensional 1H homonuclear and three-dimensional 1H- inverted question mark15N inverted question mark heteronuclear NMR spectroscopy using 785 NOE distance restraints and 47 angular restraints. The molecule folds into one globular domain that consists of two helices and nine beta-strands in two beta-sheets. The structure confirms the proposed existence of five disulfide bonds. The global fold of SRAFP is homologous to C-type lectins and pancreatic stone proteins, even though the sequence identity is only approximately 20%.
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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2AFP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Hemitripterus_americanus Hemitripterus americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AFP OCA].
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</div>
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<div class="pdbe-citations 2afp" style="background-color:#fffaf0;"></div>
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==Reference==
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==See Also==
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The solution structure of type II antifreeze protein reveals a new member of the lectin family., Gronwald W, Loewen MC, Lix B, Daugulis AJ, Sonnichsen FD, Davies PL, Sykes BD, Biochemistry. 1998 Apr 7;37(14):4712-21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9537986 9537986]
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*[[Antifreeze protein 3D structures|Antifreeze protein 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Hemitripterus americanus]]
[[Category: Hemitripterus americanus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Daugulis, A J.]]
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[[Category: Daugulis AJ]]
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[[Category: Davies, P L.]]
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[[Category: Davies PL]]
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[[Category: Gronwald, W.]]
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[[Category: Gronwald W]]
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[[Category: Lix, B.]]
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[[Category: Lix B]]
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[[Category: Loewen, M C.]]
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[[Category: Loewen MC]]
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[[Category: Sonnichsen, F D.]]
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[[Category: Sonnichsen FD]]
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[[Category: Sykes, B D.]]
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[[Category: Sykes BD]]
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[[Category: C-type lectin]]
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[[Category: Recombinant sea raven protein]]
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[[Category: Solution backbone fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:59:48 2008''
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Current revision

THE SOLUTION STRUCTURE OF TYPE II ANTIFREEZE PROTEIN REVEALS A NEW MEMBER OF THE LECTIN FAMILY

PDB ID 2afp

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