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2ane

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[[Image:2ane.gif|left|200px]]
 
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==Crystal structure of N-terminal domain of E.Coli Lon Protease==
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The line below this paragraph, containing "STRUCTURE_2ane", creates the "Structure Box" on the page.
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<StructureSection load='2ane' size='340' side='right'caption='[[2ane]], [[Resolution|resolution]] 2.03&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2ane]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ANE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ANE FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.03&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ane FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ane OCA], [https://pdbe.org/2ane PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ane RCSB], [https://www.ebi.ac.uk/pdbsum/2ane PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ane ProSAT]</span></td></tr>
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{{STRUCTURE_2ane| PDB=2ane | SCENE= }}
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</table>
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== Function ==
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'''Crystal structure of N-terminal domain of E.Coli Lon Protease'''
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[https://www.uniprot.org/uniprot/LON_ECOLI LON_ECOLI] ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins, including some antitoxins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. Degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long. Binds to DNA in a double-stranded, site-specific manner. Endogenous substrates include the regulatory proteins RcsA and SulA, the transcriptional activator SoxS, and UmuD. Its overproduction specifically inhibits translation through at least two different pathways, one of them being the YoeB-YefM toxin-antitoxin system.<ref>PMID:12135363</ref> <ref>PMID:16584195</ref> <ref>PMID:19721064</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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We report here the first crystal structure of the N-terminal domain of an A-type Lon protease. Lon proteases are ubiquitous, multidomain, ATP-dependent enzymes with both highly specific and non-specific protein binding, unfolding, and degrading activities. We expressed and purified a stable, monomeric 119-amino acid N-terminal subdomain of the Escherichia coli A-type Lon protease and determined its crystal structure at 2.03 A (Protein Data Bank [PDB] code 2ANE). The structure was solved in two crystal forms, yielding 14 independent views. The domain exhibits a unique fold consisting primarily of three twisted beta-sheets and a single long alpha-helix. Analysis of recent PDB depositions identified a similar fold in BPP1347 (PDB code 1ZBO), a 203-amino acid protein of unknown function from Bordetella parapertussis, crystallized as part of a structural genomics effort. BPP1347 shares sequence homology with Lon N-domains and with a family of other independently expressed proteins of unknown functions. We postulate that, as is the case in Lon proteases, this structural domain represents a general protein and polypeptide interaction domain.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/an/2ane_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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2ANE is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ANE OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ane ConSurf].
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Crystal structure of the N-terminal domain of E. coli Lon protease., Li M, Rasulova F, Melnikov EE, Rotanova TV, Gustchina A, Maurizi MR, Wlodawer A, Protein Sci. 2005 Nov;14(11):2895-900. Epub 2005 Sep 30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16199667 16199667]
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<div style="clear:both"></div>
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[[Category: Endopeptidase La]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Gustchina, A.]]
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[[Category: Gustchina A]]
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[[Category: Li, M.]]
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[[Category: Li M]]
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[[Category: Maurizi, M R.]]
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[[Category: Maurizi MR]]
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[[Category: Melnikov, E E.]]
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[[Category: Melnikov EE]]
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[[Category: Rasulova, F.]]
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[[Category: Rasulova F]]
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[[Category: Rotanova, T V.]]
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[[Category: Rotanova TV]]
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[[Category: Wlodawer, A.]]
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[[Category: Wlodawer A]]
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[[Category: Lon protease]]
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[[Category: Lonn119]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:15:05 2008''
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Current revision

Crystal structure of N-terminal domain of E.Coli Lon Protease

PDB ID 2ane

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