2aor

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[[Image:2aor.gif|left|200px]]
 
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==Crystal structure of MutH-hemimethylated DNA complex==
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The line below this paragraph, containing "STRUCTURE_2aor", creates the "Structure Box" on the page.
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<StructureSection load='2aor' size='340' side='right'caption='[[2aor]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2aor]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AOR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AOR FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6MA:N6-METHYL-DEOXY-ADENOSINE-5-MONOPHOSPHATE'>6MA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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{{STRUCTURE_2aor| PDB=2aor | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2aor FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aor OCA], [https://pdbe.org/2aor PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2aor RCSB], [https://www.ebi.ac.uk/pdbsum/2aor PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2aor ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MUTH_HAEIN MUTH_HAEIN] Sequence-specific endonuclease that cleaves unmethylated GATC sequences. It is involved in DNA mismatch repair (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ao/2aor_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2aor ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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MutH initiates mismatch repair by nicking the transiently unmethylated daughter strand 5' to a GATC sequence. Here, we report crystal structures of MutH complexed with hemimethylated and unmethylated GATC substrates. Both structures contain two Ca2+ ions jointly coordinated by a conserved aspartate and the scissile phosphate, as observed in the restriction endonucleases BamHI and BglI. In the hemimethylated complexes, the active site is more compact and DNA cleavage is more efficient. The Lys residue in the conserved DEK motif coordinates the nucleophilic water in conjunction with the phosphate 3' to the scissile bond; the same Lys is also hydrogen bonded with a carbonyl oxygen in the DNA binding module. We propose that this Lys, which is conserved in many restriction endonucleases and is replaced by Glu or Gln in BamHI and BglII, is a sensor for DNA binding and the linchpin that couples base recognition and DNA cleavage.
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'''Crystal structure of MutH-hemimethylated DNA complex'''
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MutH complexed with hemi- and unmethylated DNAs: coupling base recognition and DNA cleavage.,Lee JY, Chang J, Joseph N, Ghirlando R, Rao DN, Yang W Mol Cell. 2005 Oct 7;20(1):155-66. PMID:16209953<ref>PMID:16209953</ref>
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==Overview==
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MutH initiates mismatch repair by nicking the transiently unmethylated daughter strand 5' to a GATC sequence. Here, we report crystal structures of MutH complexed with hemimethylated and unmethylated GATC substrates. Both structures contain two Ca2+ ions jointly coordinated by a conserved aspartate and the scissile phosphate, as observed in the restriction endonucleases BamHI and BglI. In the hemimethylated complexes, the active site is more compact and DNA cleavage is more efficient. The Lys residue in the conserved DEK motif coordinates the nucleophilic water in conjunction with the phosphate 3' to the scissile bond; the same Lys is also hydrogen bonded with a carbonyl oxygen in the DNA binding module. We propose that this Lys, which is conserved in many restriction endonucleases and is replaced by Glu or Gln in BamHI and BglII, is a sensor for DNA binding and the linchpin that couples base recognition and DNA cleavage.
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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2AOR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AOR OCA].
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</div>
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<div class="pdbe-citations 2aor" style="background-color:#fffaf0;"></div>
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==Reference==
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==See Also==
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MutH complexed with hemi- and unmethylated DNAs: coupling base recognition and DNA cleavage., Lee JY, Chang J, Joseph N, Ghirlando R, Rao DN, Yang W, Mol Cell. 2005 Oct 7;20(1):155-66. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16209953 16209953]
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*[[DNA mismatch repair protein 3D structures|DNA mismatch repair protein 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Haemophilus influenzae]]
[[Category: Haemophilus influenzae]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Chang, J.]]
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[[Category: Chang J]]
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[[Category: Ghirlando, R.]]
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[[Category: Ghirlando R]]
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[[Category: Joseph, N.]]
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[[Category: Joseph N]]
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[[Category: Lee, J Y.]]
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[[Category: Lee JY]]
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[[Category: Rao, D N.]]
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[[Category: Rao DN]]
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[[Category: Yang, W.]]
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[[Category: Yang W]]
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[[Category: Gatc recognition]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:17:54 2008''
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Current revision

Crystal structure of MutH-hemimethylated DNA complex

PDB ID 2aor

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