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2av5

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[[Image:2av5.gif|left|200px]]
 
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==Crystal structure of Pyrococcus furiosus Pop5, an archaeal Ribonuclease P protein==
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The line below this paragraph, containing "STRUCTURE_2av5", creates the "Structure Box" on the page.
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<StructureSection load='2av5' size='340' side='right'caption='[[2av5]], [[Resolution|resolution]] 3.15&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2av5]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AV5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AV5 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.15&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2av5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2av5 OCA], [https://pdbe.org/2av5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2av5 RCSB], [https://www.ebi.ac.uk/pdbsum/2av5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2av5 ProSAT]</span></td></tr>
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{{STRUCTURE_2av5| PDB=2av5 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RNP2_PYRFU RNP2_PYRFU] Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. The RNA is catalytic, but its KM for pre-tRNA is 170-fold decreased in the presence of the 4 known protein subunits (Rnp1-4). The protein subunits also decrease the amount of Mg(2+) needed for activity.<ref>PMID:17053064</ref> <ref>PMID:21683084</ref> <ref>PMID:22298511</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/av/2av5_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2av5 ConSurf].
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<div style="clear:both"></div>
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'''Crystal structure of Pyrococcus furiosus Pop5, an archaeal Ribonuclease P protein'''
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==See Also==
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*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
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== References ==
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==Overview==
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<references/>
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We have used NMR spectroscopy and x-ray crystallography to determine the three-dimensional structure of PF1378 (Pfu Pop5), one of four protein subunits of archaeal RNase P that shares a homolog in the eukaryotic enzyme. RNase P is an essential and ubiquitous ribonucleoprotein enzyme required for maturation of tRNA. In bacteria, the enzyme's RNA subunit is responsible for cleaving the single-stranded 5' leader sequence of precursor tRNA molecules (pre-tRNA), whereas the protein subunit assists in substrate binding. Although in bacteria the RNase P holoenzyme consists of one large catalytic RNA and one small protein subunit, in archaea and eukarya the enzyme contains several (&gt; or =4) protein subunits, each of which lacks sequence similarity to the bacterial protein. The functional role of the proteins is poorly understood, as is the increased complexity in comparison to the bacterial enzyme. Pfu Pop5 has been directly implicated in catalysis by the observation that it pairs with PF1914 (Pfu Rpp30) to functionally reconstitute the catalytic domain of the RNA subunit. The protein adopts an alpha-beta sandwich fold highly homologous to the single-stranded RNA binding RRM domain. Furthermore, the three-dimensional arrangement of Pfu Pop5's structural elements is remarkably similar to that of the bacterial protein subunit. NMR spectra have been used to map the interaction of Pop5 with Pfu Rpp30. The data presented permit tantalizing hypotheses regarding the role of this protein subunit shared by archaeal and eukaryotic RNase P.
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2AV5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AV5 OCA].
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==Reference==
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Structure of Pfu Pop5, an archaeal RNase P protein., Wilson RC, Bohlen CJ, Foster MP, Bell CE, Proc Natl Acad Sci U S A. 2006 Jan 24;103(4):873-8. Epub 2006 Jan 17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16418270 16418270]
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[[Category: Pyrococcus furiosus]]
[[Category: Pyrococcus furiosus]]
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[[Category: Ribonuclease P]]
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[[Category: Bell CE]]
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[[Category: Single protein]]
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[[Category: Bohlen CJ]]
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[[Category: Bell, C E.]]
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[[Category: Foster MP]]
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[[Category: Bohlen, C J.]]
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[[Category: Wilson RC]]
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[[Category: Foster, M P.]]
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[[Category: Wilson, R C.]]
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[[Category: Ribonuclease p]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:30:46 2008''
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Current revision

Crystal structure of Pyrococcus furiosus Pop5, an archaeal Ribonuclease P protein

PDB ID 2av5

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