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2azo

From Proteopedia

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[[Image:2azo.jpg|left|200px]]
 
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==DNA MISMATCH REPAIR PROTEIN MUTH FROM E. COLI==
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The line below this paragraph, containing "STRUCTURE_2azo", creates the "Structure Box" on the page.
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<StructureSection load='2azo' size='340' side='right'caption='[[2azo]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2azo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AZO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AZO FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2azo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2azo OCA], [https://pdbe.org/2azo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2azo RCSB], [https://www.ebi.ac.uk/pdbsum/2azo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2azo ProSAT]</span></td></tr>
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{{STRUCTURE_2azo| PDB=2azo | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MUTH_ECOLI MUTH_ECOLI] Sequence-specific endonuclease that cleaves unmethylated GATC sequences. It is involved in DNA mismatch repair.[HAMAP-Rule:MF_00759]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/az/2azo_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2azo ConSurf].
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<div style="clear:both"></div>
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'''DNA MISMATCH REPAIR PROTEIN MUTH FROM E. COLI'''
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==See Also==
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*[[DNA mismatch repair protein 3D structures|DNA mismatch repair protein 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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MutS, MutL and MutH are the three essential proteins for initiation of methyl-directed DNA mismatch repair to correct mistakes made during DNA replication in Escherichia coli. MutH cleaves a newly synthesized and unmethylated daughter strand 5' to the sequence d(GATC) in a hemi-methylated duplex. Activation of MutH requires the recognition of a DNA mismatch by MutS and MutL. We have crystallized MutH in two space groups and solved the structures at 1.7 and 2.3 A resolution, respectively. The active site of MutH is located at an interface between two subdomains that pivot relative to one another, as revealed by comparison of the crystal structures, and this presumably regulates the nuclease activity. The relative motion of the two subdomains in MutH correlates with the position of a protruding C-terminal helix. This helix appears to act as a molecular lever through which MutS and MutL may communicate the detection of a DNA mismatch and activate MutH. With sequence homology to Sau3AI and structural similarity to PvuII endonuclease, MutH is clearly related to these enzymes by divergent evolution, and this suggests that type II restriction endonucleases evolved from a common ancestor.
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[[Category: Escherichia coli K-12]]
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[[Category: Large Structures]]
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==About this Structure==
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[[Category: Yang W]]
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2AZO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AZO OCA].
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==Reference==
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Structural basis for MutH activation in E.coli mismatch repair and relationship of MutH to restriction endonucleases., Ban C, Yang W, EMBO J. 1998 Mar 2;17(5):1526-34. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9482749 9482749]
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[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Yang, W.]]
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[[Category: Dna repair]]
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[[Category: Endonuclease]]
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[[Category: Hydrolase]]
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[[Category: Muth]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:40:11 2008''
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Current revision

DNA MISMATCH REPAIR PROTEIN MUTH FROM E. COLI

PDB ID 2azo

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