2b98

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[[Image:2b98.gif|left|200px]]
 
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==Crystal Structure of an archaeal pentameric riboflavin synthase==
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The line below this paragraph, containing "STRUCTURE_2b98", creates the "Structure Box" on the page.
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<StructureSection load='2b98' size='340' side='right'caption='[[2b98]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2b98]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B98 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2B98 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b98 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b98 OCA], [https://pdbe.org/2b98 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b98 RCSB], [https://www.ebi.ac.uk/pdbsum/2b98 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b98 ProSAT]</span></td></tr>
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{{STRUCTURE_2b98| PDB=2b98 | SCENE= }}
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</table>
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== Function ==
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'''Crystal Structure of an archaeal pentameric riboflavin synthase'''
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[https://www.uniprot.org/uniprot/RISC_METJA RISC_METJA]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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Whereas eubacterial and eukaryotic riboflavin synthases form homotrimers, archaeal riboflavin synthases from Methanocaldococcus jannaschii and Methanothermobacter thermoautrophicus are homopentamers with sequence similarity to the 6,7-dimethyl-8-ribityllumazine synthase catalyzing the penultimate step in riboflavin biosynthesis. Recently it could be shown that the complex dismutation reaction catalyzed by the pentameric M. jannaschii riboflavin synthase generates riboflavin with the same regiochemistry as observed for trimeric riboflavin synthases. Here we present crystal structures of the pentameric riboflavin synthase from M. jannaschii and its complex with the substrate analog inhibitor, 6,7-dioxo-8-ribityllumazine. The complex structure shows five active sites located between adjacent monomers of the pentamer. Each active site can accommodate two substrate analog molecules in anti-parallel orientation. The topology of the two bound ligands at the active site is well in line with the known stereochemistry of a pentacyclic adduct of 6,7-dimethyl-8-ribityllumazine that has been shown to serve as a kinetically competent intermediate. The pentacyclic intermediates of trimeric and pentameric riboflavin synthases are diastereomers.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b9/2b98_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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2B98 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B98 OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2b98 ConSurf].
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Crystal structure of an archaeal pentameric riboflavin synthase in complex with a substrate analog inhibitor: stereochemical implications., Ramsperger A, Augustin M, Schott AK, Gerhardt S, Krojer T, Eisenreich W, Illarionov B, Cushman M, Bacher A, Huber R, Fischer M, J Biol Chem. 2006 Jan 13;281(2):1224-32. Epub 2005 Nov 4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16272154 16272154]
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Methanocaldococcus jannaschii]]
[[Category: Methanocaldococcus jannaschii]]
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[[Category: Riboflavin synthase]]
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[[Category: Augustin M]]
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[[Category: Single protein]]
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[[Category: Bacher A]]
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[[Category: Augustin, M.]]
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[[Category: Cushman M]]
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[[Category: Bacher, A.]]
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[[Category: Eisenreich W]]
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[[Category: Cushman, M.]]
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[[Category: Fischer M]]
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[[Category: Eisenreich, W.]]
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[[Category: Gerhardt S]]
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[[Category: Fischer, M.]]
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[[Category: Huber R]]
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[[Category: Gerhardt, S.]]
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[[Category: Illarionov B]]
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[[Category: Huber, R.]]
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[[Category: Krojer T]]
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[[Category: Illarionov, B.]]
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[[Category: Ramsperger A]]
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[[Category: Krojer, T.]]
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[[Category: Schott AK]]
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[[Category: Ramsperger, A.]]
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[[Category: Schott, A K.]]
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[[Category: Lumazine riboflavin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:00:25 2008''
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Current revision

Crystal Structure of an archaeal pentameric riboflavin synthase

PDB ID 2b98

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