2esb

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(New page: 200px<br /> <applet load="2esb" size="450" color="white" frame="true" align="right" spinBox="true" caption="2esb, resolution 2.&Aring;" /> '''Crystal structure of ...)
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[[Image:2esb.gif|left|200px]]<br />
 
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<applet load="2esb" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2esb, resolution 2.&Aring;" />
 
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'''Crystal structure of human DUSP18'''<br />
 
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==Overview==
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==Crystal structure of human DUSP18==
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The human dual-specificity protein phosphatase 18 (DSP18) gene and its, protein product have recently been characterized. Like most DSPs, DSP18, displays dephosphorylating activity towards both phosphotyrosine and, phosphothreonine residues. However, DSP18 is distinct from other known, DSPs in terms of the existence of approximately 30 residues at the, C-terminus of the catalytic domain and an unusual optimum activity profile, at 328 K. The crystal structure of human DSP18 has been determined at 2.0, A resolution. The catalytic domain of DSP18 adopts a fold similar to that, known for other DSP structures. Although good alignments are found with, other DSPs, substantial differences are also found in the regions, surrounding the active site, suggesting that DSP18 constitutes a unique, structure with a distinct substrate specificity. Furthermore, the residues, at the C-terminus fold into two antiparallel beta-strands and participate, in extensive interactions with the catalytic domain, explaining the, thermostability of DSP18.
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<StructureSection load='2esb' size='340' side='right'caption='[[2esb]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2esb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ESB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ESB FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2esb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2esb OCA], [https://pdbe.org/2esb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2esb RCSB], [https://www.ebi.ac.uk/pdbsum/2esb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2esb ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DUS18_HUMAN DUS18_HUMAN] Can dephosphorylate single and diphosphorylated synthetic MAPK peptides, with preference for the phosphotyrosine and diphosphorylated forms over phosphothreonine. In vitro, dephosphorylates p-nitrophenyl phosphate (pNPP).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/es/2esb_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2esb ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2ESB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with ACT and EPE as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2ESB OCA].
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*[[Dual specificity phosphatase 3D structures|Dual specificity phosphatase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structure of human DSP18, a member of the dual-specificity protein tyrosine phosphatase family., Jeong DG, Cho YH, Yoon TS, Kim JH, Son JH, Ryu SE, Kim SJ, Acta Crystallogr D Biol Crystallogr. 2006 Jun;62(Pt 6):582-8. Epub 2006, May 12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16699184 16699184]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Cho, Y.H.]]
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[[Category: Cho YH]]
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[[Category: Jeong, D.G.]]
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[[Category: Jeong DG]]
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[[Category: Kim, J.H.]]
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[[Category: Kim JH]]
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[[Category: Kim, S.J.]]
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[[Category: Kim SJ]]
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[[Category: Ryu, S.E.]]
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[[Category: Ryu SE]]
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[[Category: Yoon, T.S.]]
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[[Category: Yoon TS]]
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[[Category: ACT]]
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[[Category: EPE]]
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[[Category: alpha/beta structure]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 21:54:58 2007''
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Current revision

Crystal structure of human DUSP18

PDB ID 2esb

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