2b9x

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[[Image:2b9x.gif|left|200px]]
 
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==Crystal Structure of CLA-producing fatty acid isomerase from P. acnes==
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The line below this paragraph, containing "STRUCTURE_2b9x", creates the "Structure Box" on the page.
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<StructureSection load='2b9x' size='340' side='right'caption='[[2b9x]], [[Resolution|resolution]] 2.22&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2b9x]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cutibacterium_acnes Cutibacterium acnes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B9X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2B9X FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.22&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=12P:DODECAETHYLENE+GLYCOL'>12P</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_2b9x| PDB=2b9x | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b9x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b9x OCA], [https://pdbe.org/2b9x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b9x RCSB], [https://www.ebi.ac.uk/pdbsum/2b9x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b9x ProSAT]</span></td></tr>
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</table>
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'''Crystal Structure of CLA-producing fatty acid isomerase from P. acnes'''
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== Function ==
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[https://www.uniprot.org/uniprot/Q6A8X5_CUTAK Q6A8X5_CUTAK]
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Conjugated linoleic acids (CLAs) affect body fat gain, carcinogenesis, insulin resistance, and lipid peroxidation in mammals. Several isomers of CLA exist, of which the (9Z, 11E) and (10E, 12Z) isomers have beneficial effects on human metabolism but are scarce in foods. Bacterial polyunsaturated fatty acid isomerases are promising biotechnological catalysts for CLA production. We describe six crystal structures of the Propionibacterium acnes polyunsaturated fatty acid isomerase PAI in apo- and product-bound forms. The three-domain flavoprotein has previously undescribed folds outside the FAD-binding site. Conformational changes in a hydrophobic channel toward the active site reveal a unique gating mechanism for substrate specificity. The geometry of the substrate-binding site explains the length preferences for C18 fatty acids. A catalytic mechanism for double-bond isomerization is formulated that may be altered to change substrate specificity for syntheses of rare CLAs from easily accessible precursors.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b9/2b9x_consurf.spt"</scriptWhenChecked>
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2B9X is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Propionibacterium_acnes Propionibacterium acnes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B9X OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Structure and mechanism of the Propionibacterium acnes polyunsaturated fatty acid isomerase., Liavonchanka A, Hornung E, Feussner I, Rudolph MG, Proc Natl Acad Sci U S A. 2006 Feb 21;103(8):2576-81. Epub 2006 Feb 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16477020 16477020]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2b9x ConSurf].
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[[Category: Propionibacterium acnes]]
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<div style="clear:both"></div>
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[[Category: Single protein]]
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__TOC__
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[[Category: Liavonchanka, A.]]
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</StructureSection>
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[[Category: Rudolph, M G.]]
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[[Category: Cutibacterium acnes]]
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[[Category: Conjugated linoleic acid]]
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[[Category: Large Structures]]
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[[Category: Fad]]
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[[Category: Liavonchanka A]]
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[[Category: Isomerase]]
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[[Category: Rudolph MG]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:01:47 2008''
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Current revision

Crystal Structure of CLA-producing fatty acid isomerase from P. acnes

PDB ID 2b9x

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