2bay

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[[Image:2bay.gif|left|200px]]
 
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==Crystal structure of the Prp19 U-box dimer==
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The line below this paragraph, containing "STRUCTURE_2bay", creates the "Structure Box" on the page.
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<StructureSection load='2bay' size='340' side='right'caption='[[2bay]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2bay]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BAY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BAY FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bay FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bay OCA], [https://pdbe.org/2bay PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bay RCSB], [https://www.ebi.ac.uk/pdbsum/2bay PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bay ProSAT]</span></td></tr>
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{{STRUCTURE_2bay| PDB=2bay | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PRP19_YEAST PRP19_YEAST] Involved in pre-mRNA splicing. Acts a central component of the NTC complex (or PRP19-associated complex) that associates to the spliceosome to mediate conformational rearrangement or to stabilize the structure of the spliceosome after U4 snRNA dissociation, which leads to spliceosome maturation. Involved in DNA repair.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ba/2bay_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bay ConSurf].
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<div style="clear:both"></div>
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'''Crystal structure of the Prp19 U-box dimer'''
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==See Also==
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*[[Pre-mRNA splicing factors 3D structures|Pre-mRNA splicing factors 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Prp19 is an essential splicing factor and a member of the U-box family of E3 ubiquitin ligases. Prp19 forms a tetramer via a central coiled-coil domain. Here, we show the U-box domain of Prp19 exists as a dimer within the context of the Prp19 tetramer. A high-resolution structure of the homodimeric state of the Prp19 U-box was determined by X-ray crystallography. Mutation of the U-box dimer interface abrogates U-box dimer formation and is lethal in vivo. The structure of the U-box dimer enables construction of a complete model of Prp19 providing insights into how the tetrameric protein functions as an E3 ligase. Finally, comparison of the Prp19 U-box homodimer with the heterodimeric complex of BRCA1/BARD1 RING-finger domains uncovers a common architecture for a family of oligomeric U-box and RING-finger E3 ubiquitin ligases, which has mechanistic implications for E3 ligase-mediated polyubiquitination and E4 polyubiquitin ligases.
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[[Category: Large Structures]]
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==About this Structure==
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2BAY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BAY OCA].
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==Reference==
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The Prp19 U-box crystal structure suggests a common dimeric architecture for a class of oligomeric E3 ubiquitin ligases., Vander Kooi CW, Ohi MD, Rosenberg JA, Oldham ML, Newcomer ME, Gould KL, Chazin WJ, Biochemistry. 2006 Jan 10;45(1):121-30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16388587 16388587]
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[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Chazin WJ]]
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[[Category: Chazin, W J.]]
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[[Category: Gould KL]]
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[[Category: Gould, K L.]]
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[[Category: Newcomer ME]]
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[[Category: Kooi, C W.Vander.]]
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[[Category: Ohi MD]]
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[[Category: Newcomer, M E.]]
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[[Category: Oldham ML]]
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[[Category: Ohi, M D.]]
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[[Category: Rosenberg JA]]
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[[Category: Oldham, M L.]]
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[[Category: Vander Kooi CW]]
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[[Category: Rosenberg, J A.]]
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[[Category: E3 ligase]]
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[[Category: Prp19]]
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[[Category: U-box]]
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[[Category: Ubiquitin ligase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:03:22 2008''
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Current revision

Crystal structure of the Prp19 U-box dimer

PDB ID 2bay

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