2bjw

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[[Image:2bjw.gif|left|200px]]
 
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==PspF AAA domain==
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The line below this paragraph, containing "STRUCTURE_2bjw", creates the "Structure Box" on the page.
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<StructureSection load='2bjw' size='340' side='right'caption='[[2bjw]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2bjw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BJW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BJW FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bjw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bjw OCA], [https://pdbe.org/2bjw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bjw RCSB], [https://www.ebi.ac.uk/pdbsum/2bjw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bjw ProSAT]</span></td></tr>
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{{STRUCTURE_2bjw| PDB=2bjw | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PSPF_ECOLI PSPF_ECOLI] Transcriptional activator for the phage shock protein (psp) operon (pspABCDE) and pspG gene.<ref>PMID:8606168</ref> <ref>PMID:15485810</ref> <ref>PMID:19804784</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bj/2bjw_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bjw ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Activators of bacterial sigma54-RNA polymerase holoenzyme are mechanochemical proteins that use adenosine triphosphate (ATP) hydrolysis to activate transcription. We have determined by cryogenic electron microscopy (cryo-EM) a 20 angstrom resolution structure of an activator, phage shock protein F [PspF(1-275)], which is bound to an ATP transition state analog in complex with its basal factor, sigma54. By fitting the crystal structure of PspF(1-275) at 1.75 angstroms into the EM map, we identified two loops involved in binding sigma54. Comparing enhancer-binding structures in different nucleotide states and mutational analysis led us to propose nucleotide-dependent conformational changes that free the loops for association with sigma54.
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'''PSPF AAA DOMAIN'''
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Structural insights into the activity of enhancer-binding proteins.,Rappas M, Schumacher J, Beuron F, Niwa H, Bordes P, Wigneshweraraj S, Keetch CA, Robinson CV, Buck M, Zhang X Science. 2005 Mar 25;307(5717):1972-5. PMID:15790859<ref>PMID:15790859</ref>
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==Overview==
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Activators of bacterial sigma54-RNA polymerase holoenzyme are mechanochemical proteins that use adenosine triphosphate (ATP) hydrolysis to activate transcription. We have determined by cryogenic electron microscopy (cryo-EM) a 20 angstrom resolution structure of an activator, phage shock protein F [PspF(1-275)], which is bound to an ATP transition state analog in complex with its basal factor, sigma54. By fitting the crystal structure of PspF(1-275) at 1.75 angstroms into the EM map, we identified two loops involved in binding sigma54. Comparing enhancer-binding structures in different nucleotide states and mutational analysis led us to propose nucleotide-dependent conformational changes that free the loops for association with sigma54.
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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2BJW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BJW OCA].
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</div>
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<div class="pdbe-citations 2bjw" style="background-color:#fffaf0;"></div>
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==Reference==
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==See Also==
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Structural insights into the activity of enhancer-binding proteins., Rappas M, Schumacher J, Beuron F, Niwa H, Bordes P, Wigneshweraraj S, Keetch CA, Robinson CV, Buck M, Zhang X, Science. 2005 Mar 25;307(5717):1972-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15790859 15790859]
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*[[Transcriptional activator 3D structures|Transcriptional activator 3D structures]]
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[[Category: Escherichia coli]]
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== References ==
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[[Category: Single protein]]
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<references/>
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[[Category: Beuron, F.]]
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__TOC__
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[[Category: Bordes, P.]]
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</StructureSection>
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[[Category: Buck, M.]]
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[[Category: Escherichia coli K-12]]
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[[Category: Keetch, C A.]]
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[[Category: Large Structures]]
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[[Category: Niwa, H.]]
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[[Category: Beuron F]]
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[[Category: Rappas, M.]]
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[[Category: Bordes P]]
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[[Category: Robinson, C V.]]
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[[Category: Buck M]]
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[[Category: Schumacher, J.]]
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[[Category: Keetch CA]]
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[[Category: Wigneshweraraj, S.]]
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[[Category: Niwa H]]
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[[Category: Zhang, X.]]
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[[Category: Rappas M]]
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[[Category: Aaa,transcription activation]]
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[[Category: Robinson CV]]
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[[Category: Atp-binding]]
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[[Category: Schumacher J]]
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[[Category: Dna-binding]]
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[[Category: Wigneshweraraj S]]
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[[Category: Enhancer binding protein]]
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[[Category: Zhang X]]
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[[Category: Gene regulation]]
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[[Category: Pspf]]
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[[Category: Sigma54 activator]]
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[[Category: Transcription regulation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:23:27 2008''
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Current revision

PspF AAA domain

PDB ID 2bjw

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