This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2bm5

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (06:38, 1 May 2024) (edit) (undo)
 
(10 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2bm5.gif|left|200px]]
 
-
<!--
+
==The Structure of MfpA (Rv3361c, P21 Crystal form). The Pentapeptide Repeat Protein from Mycobacterium tuberculosis Folds as A Right- handed Quadrilateral Beta-helix.==
-
The line below this paragraph, containing "STRUCTURE_2bm5", creates the "Structure Box" on the page.
+
<StructureSection load='2bm5' size='340' side='right'caption='[[2bm5]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2bm5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BM5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BM5 FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-
{{STRUCTURE_2bm5| PDB=2bm5 | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bm5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bm5 OCA], [https://pdbe.org/2bm5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bm5 RCSB], [https://www.ebi.ac.uk/pdbsum/2bm5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bm5 ProSAT]</span></td></tr>
-
 
+
</table>
-
'''THE STRUCTURE OF MFPA (RV3361C, P21 CRYSTAL FORM). THE PENTAPEPTIDE REPEAT PROTEIN FROM MYCOBACTERIUM TUBERCULOSIS FOLDS AS A RIGHT-HANDED QUADRILATERAL BETA-HELIX.'''
+
== Function ==
-
 
+
[https://www.uniprot.org/uniprot/O50390_MYCTO O50390_MYCTO]
-
 
+
== Evolutionary Conservation ==
-
==Overview==
+
[[Image:Consurf_key_small.gif|200px|right]]
-
Fluoroquinolones are gaining increasing importance in the treatment of tuberculosis. The expression of MfpA, a member of the pentapeptide repeat family of proteins from Mycobacterium tuberculosis, causes resistance to ciprofloxacin and sparfloxacin. This protein binds to DNA gyrase and inhibits its activity. Its three-dimensional structure reveals a fold, which we have named the right-handed quadrilateral beta helix, that exhibits size, shape, and electrostatic similarity to B-form DNA. This represents a form of DNA mimicry and explains both its inhibitory effect on DNA gyrase and fluoroquinolone resistance resulting from the protein's expression in vivo.
+
Check<jmol>
-
 
+
<jmolCheckbox>
-
==About this Structure==
+
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bm/2bm5_consurf.spt"</scriptWhenChecked>
-
2BM5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BM5 OCA].
+
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-
 
+
<text>to colour the structure by Evolutionary Conservation</text>
-
==Reference==
+
</jmolCheckbox>
-
A fluoroquinolone resistance protein from Mycobacterium tuberculosis that mimics DNA., Hegde SS, Vetting MW, Roderick SL, Mitchenall LA, Maxwell A, Takiff HE, Blanchard JS, Science. 2005 Jun 3;308(5727):1480-3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15933203 15933203]
+
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bm5 ConSurf].
-
[[Category: Mycobacterium tuberculosis]]
+
<div style="clear:both"></div>
-
[[Category: Single protein]]
+
__TOC__
-
[[Category: Blanchard, J S.]]
+
</StructureSection>
-
[[Category: Hegde, S S.]]
+
[[Category: Large Structures]]
-
[[Category: Maxwell, A.]]
+
[[Category: Mycobacterium tuberculosis H37Rv]]
-
[[Category: Mitchenall, L A.]]
+
[[Category: Blanchard JS]]
-
[[Category: Roderick, S L.]]
+
[[Category: Hegde SS]]
-
[[Category: Takiff, H E.]]
+
[[Category: Maxwell A]]
-
[[Category: Vetting, M W.]]
+
[[Category: Mitchenall LA]]
-
[[Category: Dna gyrase]]
+
[[Category: Roderick SL]]
-
[[Category: Dna mimicry]]
+
[[Category: Takiff HE]]
-
[[Category: Pentapeptide repeat protein,fluroquinolone resistance]]
+
[[Category: Vetting MW]]
-
[[Category: Right-handed quadrilateral beta-helix]]
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:28:47 2008''
+

Current revision

The Structure of MfpA (Rv3361c, P21 Crystal form). The Pentapeptide Repeat Protein from Mycobacterium tuberculosis Folds as A Right- handed Quadrilateral Beta-helix.

PDB ID 2bm5

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2bm5"
Personal tools