2bmj

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[[Image:2bmj.gif|left|200px]]
 
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==GTPase like domain of Centaurin Gamma 1 (Human)==
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The line below this paragraph, containing "STRUCTURE_2bmj", creates the "Structure Box" on the page.
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<StructureSection load='2bmj' size='340' side='right'caption='[[2bmj]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2bmj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BMJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BMJ FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bmj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bmj OCA], [https://pdbe.org/2bmj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bmj RCSB], [https://www.ebi.ac.uk/pdbsum/2bmj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bmj ProSAT]</span></td></tr>
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{{STRUCTURE_2bmj| PDB=2bmj | SCENE= }}
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</table>
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== Function ==
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'''GTPASE LIKE DOMAIN OF CENTAURIN GAMMA 1 (HUMAN)'''
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[https://www.uniprot.org/uniprot/AGAP2_HUMAN AGAP2_HUMAN] GTPase-activating protein (GAP) for ARF1 and ARF5, which also shows strong GTPase activity. Isoform 1 participates in the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. It also regulates the adapter protein 1 (AP-1)-dependent trafficking of proteins in the endosomal system. It seems to be oncogenic. It is overexpressed in cancer cells, prevents apoptosis and promotes cancer cell invasion.<ref>PMID:12640130</ref> <ref>PMID:14761976</ref> <ref>PMID:15118108</ref> <ref>PMID:16079295</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bm/2bmj_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bmj ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
Centaurins are a family of proteins that contain GTPase-activating protein domains, with the gamma family members containing in addition a GTPase-like domain. Centaurins reside mainly in the nucleus and are known to activate phosphoinositide 3-kinase, a key regulator of cell proliferation, motility and vesicular trafficking. In the present study, using X-ray structural analysis, enzymatic assays and nucleotide-binding studies, we show that, for CENTG1 (centaurin gamma-1) the GTPase-like domain has broader trinucleotide specificity. Alterations within the G4 motif of CENTG1 from the highly conserved NKXD found in typical GTPases to TQDR result in the loss of specificity, a lower affinity for the nucleotides and higher turnover rates. These results indicate that the centaurins could be more accurately classified as NTPases and point to alternative mechanisms of cell signalling control.
Centaurins are a family of proteins that contain GTPase-activating protein domains, with the gamma family members containing in addition a GTPase-like domain. Centaurins reside mainly in the nucleus and are known to activate phosphoinositide 3-kinase, a key regulator of cell proliferation, motility and vesicular trafficking. In the present study, using X-ray structural analysis, enzymatic assays and nucleotide-binding studies, we show that, for CENTG1 (centaurin gamma-1) the GTPase-like domain has broader trinucleotide specificity. Alterations within the G4 motif of CENTG1 from the highly conserved NKXD found in typical GTPases to TQDR result in the loss of specificity, a lower affinity for the nucleotides and higher turnover rates. These results indicate that the centaurins could be more accurately classified as NTPases and point to alternative mechanisms of cell signalling control.
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==About this Structure==
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The centaurin gamma-1 GTPase-like domain functions as an NTPase.,Soundararajan M, Yang X, Elkins JM, Sobott F, Doyle DA Biochem J. 2007 Feb 1;401(3):679-88. PMID:17037982<ref>PMID:17037982</ref>
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2BMJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BMJ OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The centaurin gamma-1 GTPase-like domain functions as an NTPase., Soundararajan M, Yang X, Elkins JM, Sobott F, Doyle DA, Biochem J. 2007 Feb 1;401(3):679-88. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17037982 17037982]
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</div>
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<div class="pdbe-citations 2bmj" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Arrowsmith, C.]]
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[[Category: Arrowsmith C]]
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[[Category: Doyle, D A.]]
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[[Category: Doyle DA]]
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[[Category: Edwards, A.]]
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[[Category: Edwards A]]
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[[Category: Elkins, J M.]]
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[[Category: Elkins JM]]
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[[Category: Sgc, Structural Genomics Consortium.]]
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[[Category: Soundararajan M]]
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[[Category: Soundararajan, M.]]
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[[Category: Sundstrom M]]
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[[Category: Sundstrom, M.]]
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[[Category: Yang X]]
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[[Category: Yang, X.]]
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[[Category: Centaurin ank repeat]]
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[[Category: Gtp-binding]]
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[[Category: Gtpase]]
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[[Category: Hydrolase]]
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[[Category: Sgc]]
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[[Category: Structural genomics consortium]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:29:56 2008''
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Current revision

GTPase like domain of Centaurin Gamma 1 (Human)

PDB ID 2bmj

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