This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2bn6

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (13:35, 30 August 2023) (edit) (undo)
 
(9 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2bn6.gif|left|200px]]
 
-
<!--
+
==P-Element Somatic Inhibitor Protein==
-
The line below this paragraph, containing "STRUCTURE_2bn6", creates the "Structure Box" on the page.
+
<StructureSection load='2bn6' size='340' side='right'caption='[[2bn6]]' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2bn6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BN6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BN6 FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-
-->
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bn6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bn6 OCA], [https://pdbe.org/2bn6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bn6 RCSB], [https://www.ebi.ac.uk/pdbsum/2bn6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bn6 ProSAT]</span></td></tr>
-
{{STRUCTURE_2bn6| PDB=2bn6 | SCENE= }}
+
</table>
-
 
+
== Function ==
-
'''P-ELEMENT SOMATIC INHIBITOR PROTEIN'''
+
[https://www.uniprot.org/uniprot/Q7JPS0_DROSP Q7JPS0_DROSP]
-
 
+
<div style="background-color:#fffaf0;">
-
 
+
== Publication Abstract from PubMed ==
-
==Overview==
+
P-element transposition in Drosophila is regulated by tissue-specific alternative splicing of the P-element transposase pre-mRNA. In somatic cells, the P-element somatic inhibitor (PSI) protein binds to exon 3 of the pre-mRNA and recruits U1 small nuclear ribonucleoprotein (snRNP) to the F1 pseudo-splice site. This abrogates binding of U1 snRNP to the genuine 5' splice site, thereby preventing excision of the third intron. Two homologous short sequences, referred to as the A and B boxes, near the C terminus of PSI bind to U1-70k protein within U1 snRNP. We have now mapped the AB box-binding site of U1-70k to a short proline-rich sequence at the C terminus. Our NMR study shows that the B box forms an anti-parallel helical hairpin in which four highly conserved aromatic residues form a cluster on one face of the first helix. This hydrophobic cluster interacts extensively with the proline-rich region of the U1-70k protein.
P-element transposition in Drosophila is regulated by tissue-specific alternative splicing of the P-element transposase pre-mRNA. In somatic cells, the P-element somatic inhibitor (PSI) protein binds to exon 3 of the pre-mRNA and recruits U1 small nuclear ribonucleoprotein (snRNP) to the F1 pseudo-splice site. This abrogates binding of U1 snRNP to the genuine 5' splice site, thereby preventing excision of the third intron. Two homologous short sequences, referred to as the A and B boxes, near the C terminus of PSI bind to U1-70k protein within U1 snRNP. We have now mapped the AB box-binding site of U1-70k to a short proline-rich sequence at the C terminus. Our NMR study shows that the B box forms an anti-parallel helical hairpin in which four highly conserved aromatic residues form a cluster on one face of the first helix. This hydrophobic cluster interacts extensively with the proline-rich region of the U1-70k protein.
-
==About this Structure==
+
Structural basis of the interaction between P-element somatic inhibitor and U1-70k essential for the alternative splicing of P-element transposase.,Ignjatovic T, Yang JC, Butler J, Neuhaus D, Nagai K J Mol Biol. 2005 Aug 5;351(1):52-65. PMID:15990112<ref>PMID:15990112</ref>
-
2BN6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BN6 OCA].
+
-
==Reference==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
Structural basis of the interaction between P-element somatic inhibitor and U1-70k essential for the alternative splicing of P-element transposase., Ignjatovic T, Yang JC, Butler J, Neuhaus D, Nagai K, J Mol Biol. 2005 Aug 5;351(1):52-65. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15990112 15990112]
+
</div>
 +
<div class="pdbe-citations 2bn6" style="background-color:#fffaf0;"></div>
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
[[Category: Drosophila melanogaster]]
[[Category: Drosophila melanogaster]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Butler, P J.G.]]
+
[[Category: Butler PJG]]
-
[[Category: Ignjatovic, T.]]
+
[[Category: Ignjatovic T]]
-
[[Category: Nagai, K.]]
+
[[Category: Nagai K]]
-
[[Category: Neuhaus, D.]]
+
[[Category: Neuhaus D]]
-
[[Category: Yang, J C.]]
+
[[Category: Yang JC]]
-
[[Category: Nmr structure]]
+
-
[[Category: Nuclear protein]]
+
-
[[Category: Proline-rich peptide]]
+
-
[[Category: Protein-protein interaction]]
+
-
[[Category: Splicing]]
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:31:20 2008''
+

Current revision

P-Element Somatic Inhibitor Protein

PDB ID 2bn6

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2bn6"
Personal tools