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2f7k

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(New page: 200px<br /> <applet load="2f7k" size="450" color="white" frame="true" align="right" spinBox="true" caption="2f7k, resolution 2.8&Aring;" /> '''Crystal Structure of...)
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[[Image:2f7k.gif|left|200px]]<br />
 
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<applet load="2f7k" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2f7k, resolution 2.8&Aring;" />
 
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'''Crystal Structure of Human Pyridoxal Kinase'''<br />
 
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==Overview==
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==Crystal Structure of Human Pyridoxal Kinase==
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Pyridoxal kinase, a member of the ribokinase superfamily, catalyzes the, ATP-dependent phosphorylation reaction of vitamin B6 and is an essential, enzyme in the formation of pyridoxal-5'-phosphate, a key cofactor for over, 100 enzymes. Pyridoxal kinase is thus regarded as a potential target for, pharmacological agents. In this paper, we report the 2.8 angstroms crystal, structure of human pyridoxal kinase (HPLK) expressed in Escherichia coli., The diffraction data revealed unexpected merohedral perfect twinning along, the crystallographic c axis. Taking perfect twinning into account, the, structure in dimeric form was well refined according to the CNS program., Structure comparison reveals that the key 12-residue peptide over the, active site in HPLK is a beta-strand/loop/beta-strand flap, while the, corresponding peptide in sheep brain enzyme adopts a loop conformation., Moreover, HPLK possesses a more hydrophobic ATP-binding pocket. This, structure will facilitate further biochemical studies and structure-based, design of drugs related to pyridoxal kinase.
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<StructureSection load='2f7k' size='340' side='right'caption='[[2f7k]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2f7k]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F7K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2F7K FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2f7k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f7k OCA], [https://pdbe.org/2f7k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2f7k RCSB], [https://www.ebi.ac.uk/pdbsum/2f7k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2f7k ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PDXK_HUMAN PDXK_HUMAN] Required for synthesis of pyridoxal-5-phosphate from vitamin B6.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f7/2f7k_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2f7k ConSurf].
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<div style="clear:both"></div>
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==Disease==
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==See Also==
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Known diseases associated with this structure: Paroxysmal nonkinesigenic dyskinesia OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=609023 609023]], Polycystic kidney and hepatic disease OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=606702 606702]]
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*[[Pyridoxal kinase|Pyridoxal kinase]]
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__TOC__
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==About this Structure==
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</StructureSection>
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2F7K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Active as [http://en.wikipedia.org/wiki/Pyridoxal_kinase Pyridoxal kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.35 2.7.1.35] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2F7K OCA].
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==Reference==
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Crystal structure of human pyridoxal kinase., Cao P, Gong Y, Tang L, Leung YC, Jiang T, J Struct Biol. 2006 Jun;154(3):327-32. Epub 2006 Mar 20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16600635 16600635]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Pyridoxal kinase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Cao P]]
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[[Category: Cao, P.]]
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[[Category: Gong Y]]
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[[Category: Gong, Y.]]
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[[Category: Jiang T]]
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[[Category: Jiang, T.]]
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[[Category: Tang L]]
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[[Category: Tang, L.]]
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[[Category: alpha-beta structure]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 22:00:50 2007''
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Current revision

Crystal Structure of Human Pyridoxal Kinase

PDB ID 2f7k

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