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| - | [[Image:2bpl.gif|left|200px]] | + | #REDIRECT [[4amv]] This PDB entry is obsolete and replaced by 4amv |
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| - | {{STRUCTURE_2bpl| PDB=2bpl | SCENE= }}
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| - | '''E.COLI GLUCOSAMINE-6P SYNTHASE IN COMPLEX WITH FRUCTOSE-6P'''
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| - | ==Overview==
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| - | Glucosamine-6P synthase catalyzes the synthesis of glucosamine-6P from fructose-6P and glutamine and uses a channel to transfer ammonia from its glutaminase to its synthase active site. X-ray structures of glucosamine-6P synthase have been determined at 2.05 Angstroms resolution in the presence of fructose-6P and at 2.35 Angstroms resolution in the presence of fructose-6P and 6-diazo-5-oxo-L-norleucine, a glutamine affinity analog that covalently modifies the N-terminal catalytic cysteine, therefore mimicking the gamma-glutamyl-thioester intermediate formed during hydrolysis of glutamine. The fixation of the glutamine analog activates the enzyme through several major structural changes: 1) the closure of a loop to shield the glutaminase site accompanied by significant domain hinging, 2) the activation of catalytic residues involved in glutamine hydrolysis, i.e. the alpha-amino group of Cys-1 and Asn-98 that is positioned to form the oxyanion hole, and 3) a 75 degrees rotation of the Trp-74 indole group that opens the ammonia channel.
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| - | ==About this Structure==
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| - | 2BPL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BPL OCA].
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| - | ==Reference==
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| - | Glutamine binding opens the ammonia channel and activates glucosamine-6P synthase., Mouilleron S, Badet-Denisot MA, Golinelli-Pimpaneau B, J Biol Chem. 2006 Feb 17;281(7):4404-12. Epub 2005 Dec 9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16339762 16339762]
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| - | [[Category: Escherichia coli]]
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| - | [[Category: Single protein]]
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| - | [[Category: Golinelli-Pimpaneau, B.]]
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| - | [[Category: Mouilleron, S.]]
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| - | [[Category: Amidotransferase]]
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| - | [[Category: Ammonia channeling]]
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| - | [[Category: Fructose 6-phosphate]]
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| - | [[Category: Glucosamine 6-phosphate synthase]]
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| - | [[Category: Glutamine amidotransferase]]
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| - | [[Category: N terminal nucleophile]]
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| - | [[Category: Transferase]]
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:37:06 2008''
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