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2ffq

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(New page: 200px<br /> <applet load="2ffq" size="450" color="white" frame="true" align="right" spinBox="true" caption="2ffq, resolution 1.78&Aring;" /> '''The crystal structu...)
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[[Image:2ffq.gif|left|200px]]<br />
 
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<applet load="2ffq" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2ffq, resolution 1.78&Aring;" />
 
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'''The crystal structure of human neuronal Rab6B in its active GTPgS-bound form'''<br />
 
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==Overview==
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==The crystal structure of human neuronal Rab6B in its active GTPgS-bound form==
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The Rab small G-protein family plays important roles in eukaryotes as, regulators of vesicle traffic. In Rab proteins, the hydrolysis of GTP to, GDP is coupled with association with and dissociation from membranes., Conformational changes related to their different nucleotide states, determine their effector specificity. The crystal structure of human, neuronal Rab6B was solved in its 'inactive' (with bound MgGDP) and, 'active' (MgGTPgammaS-bound) forms to 2.3 and 1.8 A, respectively. Both, crystallized in space group P2(1)2(1)2(1), with similar unit-cell, parameters, allowing the comparison of both structures without packing, artifacts. Conformational changes between the inactive GDP and active, GTP-like state are observed mainly in the switch I and switch II regions, confirming their role as a molecular switch. Compared with other Rab, proteins, additional changes are observed in the Rab6 subfamily-specific, RabSF3 region that might contribute to the specificity of Rab6 for its, different effector proteins.
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<StructureSection load='2ffq' size='340' side='right'caption='[[2ffq]], [[Resolution|resolution]] 1.78&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2ffq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FFQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FFQ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.78&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GSP:5-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE'>GSP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ffq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ffq OCA], [https://pdbe.org/2ffq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ffq RCSB], [https://www.ebi.ac.uk/pdbsum/2ffq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ffq ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RAB6B_HUMAN RAB6B_HUMAN] Seems to have a role in retrograde membrane traffic at the level of the Golgi complex. May function in retrograde transport in neuronal cells.<ref>PMID:17707369</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ff/2ffq_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ffq ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2FFQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with MG and GSP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2FFQ OCA].
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*[[Ras-related protein Rab 3D structures|Ras-related protein Rab 3D structures]]
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== References ==
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==Reference==
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<references/>
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The structure of human neuronal Rab6B in the active and inactive form., Garcia-Saez I, Tcherniuk S, Kozielski F, Acta Crystallogr D Biol Crystallogr. 2006 Jul;62(Pt 7):725-33. Epub 2006, Jun 20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16790928 16790928]
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Garcia-Saez, I.]]
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[[Category: Garcia-Saez I]]
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[[Category: Kozielski, F.]]
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[[Category: Kozielski F]]
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[[Category: Tcherniuk, S.]]
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[[Category: Tcherniuk S]]
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[[Category: GSP]]
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[[Category: MG]]
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[[Category: protein-nucleotide complex]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 22:04:36 2007''
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Current revision

The crystal structure of human neuronal Rab6B in its active GTPgS-bound form

PDB ID 2ffq

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