2c7j

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[[Image:2c7j.gif|left|200px]]
 
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==Phycoerythrocyanin from Mastigocladus laminosus, 295 K, 3.0 A==
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The line below this paragraph, containing "STRUCTURE_2c7j", creates the "Structure Box" on the page.
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<StructureSection load='2c7j' size='340' side='right'caption='[[2c7j]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2c7j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mastigocladus_laminosus Mastigocladus laminosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C7J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2C7J FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BLA:BILIVERDINE+IX+ALPHA'>BLA</scene>, <scene name='pdbligand=CYC:PHYCOCYANOBILIN'>CYC</scene></td></tr>
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{{STRUCTURE_2c7j| PDB=2c7j | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2c7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c7j OCA], [https://pdbe.org/2c7j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2c7j RCSB], [https://www.ebi.ac.uk/pdbsum/2c7j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2c7j ProSAT]</span></td></tr>
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</table>
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'''PHYCOERYTHROCYANIN FROM MASTIGOCLADUS LAMINOSUS, 295 K, 3.0 A'''
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== Function ==
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[https://www.uniprot.org/uniprot/PHEA_MASLA PHEA_MASLA] Light-harvesting photosynthetic bile pigment-protein from the phycobiliprotein complex.
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c7/2c7j_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2c7j ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
Phycoerythrocyanin is the only cyanobacterial phycobiliprotein containing phycoviolobilin as a chromophore. The phycoviolobilin chromophore is photo-reactive; upon irradiation, the chromophore undergoes a Z/E-isomerization involving the rotation of pyrrole-ring D. We have determined the structure of trimeric phycoerythrocyanin at three different experimental settings: monochromatically at 110 K and 295 K as well as with the Laue method at 288 K. Based on their chemical structures, the restraints for the phycoviolobilin of the alpha-subunit and for the phycocyanobilin chromophores of the beta-subunit were newly generated, which allows a chemically meaningful refinement of both chromophores. All three phycoerythrocyanin structures are very similar; the subunits match within 0.5 A. The detailed comparison of the data obtained with the different measurements provided information about the protein properties around the phycoviolobilin chromophore. For the first time, crystals of a phycobilisome protein are used successfully with the Laue technique. This paves the way for time-resolved macromolecular crystallography, which is able to elucidate the exact mechanisms of the phycoviolobilin photoactivity including the protein involvement.
Phycoerythrocyanin is the only cyanobacterial phycobiliprotein containing phycoviolobilin as a chromophore. The phycoviolobilin chromophore is photo-reactive; upon irradiation, the chromophore undergoes a Z/E-isomerization involving the rotation of pyrrole-ring D. We have determined the structure of trimeric phycoerythrocyanin at three different experimental settings: monochromatically at 110 K and 295 K as well as with the Laue method at 288 K. Based on their chemical structures, the restraints for the phycoviolobilin of the alpha-subunit and for the phycocyanobilin chromophores of the beta-subunit were newly generated, which allows a chemically meaningful refinement of both chromophores. All three phycoerythrocyanin structures are very similar; the subunits match within 0.5 A. The detailed comparison of the data obtained with the different measurements provided information about the protein properties around the phycoviolobilin chromophore. For the first time, crystals of a phycobilisome protein are used successfully with the Laue technique. This paves the way for time-resolved macromolecular crystallography, which is able to elucidate the exact mechanisms of the phycoviolobilin photoactivity including the protein involvement.
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==About this Structure==
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Local protein flexibility as a prerequisite for reversible chromophore isomerization in alpha-phycoerythrocyanin.,Schmidt M, Krasselt A, Reuter W Biochim Biophys Acta. 2006 Jan;1764(1):55-62. Epub 2005 Nov 21. PMID:16377266<ref>PMID:16377266</ref>
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2C7J is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mastigocladus_laminosus Mastigocladus laminosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C7J OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Local protein flexibility as a prerequisite for reversible chromophore isomerization in alpha-phycoerythrocyanin., Schmidt M, Krasselt A, Reuter W, Biochim Biophys Acta. 2006 Jan;1764(1):55-62. Epub 2005 Nov 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16377266 16377266]
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</div>
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<div class="pdbe-citations 2c7j" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mastigocladus laminosus]]
[[Category: Mastigocladus laminosus]]
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[[Category: Protein complex]]
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[[Category: Krasselt A]]
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[[Category: Krasselt, A.]]
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[[Category: Reuter W]]
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[[Category: Reuter, W.]]
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[[Category: Schmidt M]]
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[[Category: Schmidt, M.]]
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[[Category: Bile pigment]]
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[[Category: Chromophore]]
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[[Category: Electron transport]]
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[[Category: Photosynthesis]]
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[[Category: Phycobilisome]]
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[[Category: Phycocyanobilin]]
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[[Category: Phycoerythrocyanin]]
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[[Category: Phycoviolobilin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 21:23:06 2008''
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Current revision

Phycoerythrocyanin from Mastigocladus laminosus, 295 K, 3.0 A

PDB ID 2c7j

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