2ccz

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (14:13, 13 December 2023) (edit) (undo)
 
(10 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2ccz.gif|left|200px]]
 
-
<!--
+
==Crystal structure of E. coli primosomol protein PriB bound to ssDNA==
-
The line below this paragraph, containing "STRUCTURE_2ccz", creates the "Structure Box" on the page.
+
<StructureSection load='2ccz' size='340' side='right'caption='[[2ccz]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2ccz]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CCZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CCZ FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
-
-->
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ccz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ccz OCA], [https://pdbe.org/2ccz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ccz RCSB], [https://www.ebi.ac.uk/pdbsum/2ccz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ccz ProSAT]</span></td></tr>
-
{{STRUCTURE_2ccz| PDB=2ccz | SCENE= }}
+
</table>
-
 
+
== Function ==
-
'''CRYSTAL STRUCTURE OF E. COLI PRIMOSOMOL PROTEIN PRIB BOUND TO SSDNA'''
+
[https://www.uniprot.org/uniprot/PRIB_ECOLI PRIB_ECOLI] Binds single-stranded DNA at the primosome assembly site (PAS). During primosome assembly it facilitates the complex formation between PriA and DnaT.<ref>PMID:1856227</ref> <ref>PMID:1646811</ref> <ref>PMID:8366072</ref>
-
 
+
== Evolutionary Conservation ==
-
 
+
[[Image:Consurf_key_small.gif|200px|right]]
-
==Overview==
+
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cc/2ccz_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ccz ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
PriB is a primosomal protein required for replication restart in Escherichia coli. PriB stimulates PriA helicase activity via interaction with single-stranded DNA (ssDNA), but the molecular details of this interaction remain unclear. Here, we report the crystal structure of PriB complexed with a 15 bases oligonucleotide (dT15) at 2.7 A resolution. PriB shares structural similarity with the E.coli ssDNA-binding protein (EcoSSB). However, the structure of the PriB-dT15 complex reveals that PriB binds ssDNA differently. Results from filter-binding assays show that PriB-ssDNA interaction is salt-sensitive and cooperative. Mutational analysis suggests that the loop L45 plays an important role in ssDNA binding. Based on the crystal structure and biochemical analyses, we propose a cooperative mechanism for the binding of PriB to ssDNA and a model for the assembly of the PriA-PriB-ssDNA complex. This report presents the first structure of a replication restart primosomal protein complexed with DNA, and a novel model that explains the interactions between a dimeric oligonucleotide-binding-fold protein and ssDNA.
PriB is a primosomal protein required for replication restart in Escherichia coli. PriB stimulates PriA helicase activity via interaction with single-stranded DNA (ssDNA), but the molecular details of this interaction remain unclear. Here, we report the crystal structure of PriB complexed with a 15 bases oligonucleotide (dT15) at 2.7 A resolution. PriB shares structural similarity with the E.coli ssDNA-binding protein (EcoSSB). However, the structure of the PriB-dT15 complex reveals that PriB binds ssDNA differently. Results from filter-binding assays show that PriB-ssDNA interaction is salt-sensitive and cooperative. Mutational analysis suggests that the loop L45 plays an important role in ssDNA binding. Based on the crystal structure and biochemical analyses, we propose a cooperative mechanism for the binding of PriB to ssDNA and a model for the assembly of the PriA-PriB-ssDNA complex. This report presents the first structure of a replication restart primosomal protein complexed with DNA, and a novel model that explains the interactions between a dimeric oligonucleotide-binding-fold protein and ssDNA.
-
==About this Structure==
+
Complexed crystal structure of replication restart primosome protein PriB reveals a novel single-stranded DNA-binding mode.,Huang CY, Hsu CH, Sun YJ, Wu HN, Hsiao CD Nucleic Acids Res. 2006;34(14):3878-86. Epub 2006 Aug 9. PMID:16899446<ref>PMID:16899446</ref>
-
2CCZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CCZ OCA].
+
-
==Reference==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
Complexed crystal structure of replication restart primosome protein PriB reveals a novel single-stranded DNA-binding mode., Huang CY, Hsu CH, Sun YJ, Wu HN, Hsiao CD, Nucleic Acids Res. 2006;34(14):3878-86. Epub 2006 Aug 9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16899446 16899446]
+
</div>
-
[[Category: Escherichia coli]]
+
<div class="pdbe-citations 2ccz" style="background-color:#fffaf0;"></div>
-
[[Category: Single protein]]
+
== References ==
-
[[Category: Hsiao, C D.]]
+
<references/>
-
[[Category: Hsu, C H.]]
+
__TOC__
-
[[Category: Huang, C Y.]]
+
</StructureSection>
-
[[Category: Sun, Y J.]]
+
[[Category: Escherichia coli K-12]]
-
[[Category: Wu, H N.]]
+
[[Category: Large Structures]]
-
[[Category: Primosome,prib,dna replication,dna repair,dna recombination,ssdna,single-stranded dna,dna/protein complex]]
+
[[Category: Hsiao C-D]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 21:50:45 2008''
+
[[Category: Hsu C-H]]
 +
[[Category: Huang C-Y]]
 +
[[Category: Sun Y-J]]
 +
[[Category: Wu H-N]]

Current revision

Crystal structure of E. coli primosomol protein PriB bound to ssDNA

PDB ID 2ccz

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Personal tools