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2fze

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(New page: 200px<br /> <applet load="2fze" size="450" color="white" frame="true" align="right" spinBox="true" caption="2fze, resolution 1.900&Aring;" /> '''Crystal structure ...)
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[[Image:2fze.gif|left|200px]]<br />
 
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<applet load="2fze" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2fze, resolution 1.900&Aring;" />
 
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'''Crystal structure of the binary complex of human glutathione-dependent formaldehyde dehydrogenase with ADP-ribose'''<br />
 
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==Overview==
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==Crystal structure of the binary complex of human glutathione-dependent formaldehyde dehydrogenase with ADP-ribose==
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The active-site zinc in human glutathione-dependent formaldehyde, dehydrogenase (FDH) undergoes coenzyme-induced displacement and transient, coordination to a highly conserved glutamate residue (Glu-67) during the, catalytic cycle. The role of this transient coordination of the, active-site zinc to Glu-67 in the FDH catalytic cycle and the associated, coenzyme interactions were investigated by studying enzymes in which, Glu-67 and Arg-368 were substituted with Leu. Structures of FDH.adenosine, 5'-diphosphate ribose (ADP-ribose) and E67L.NAD(H) binary complexes were, determined. Steady-state kinetics, isotope effects, and presteady-state, analysis of the E67L enzyme show that Glu-67 is critical for capturing the, substrates for catalysis. The catalytic efficiency (V/K(m)) of the E67L, enzyme in reactions involving S-nitrosoglutathione (GSNO), S-hydroxymethylglutathione (HMGSH) and 12-hydroxydodecanoic acid (12-HDDA), were 25 000-, 3000-, and 180-fold lower, respectively, than for the, wild-type enzyme. The large decrease in the efficiency of capturing GSNO, and HMGSH by the E67L enzyme results mainly because of the impaired, binding of these substrates to the mutant enzyme. In the case of 12-HDDA, a decrease in the rate of hydride transfer is the major factor responsible, for the reduction in the efficiency of its capture for catalysis by the, E67L enzyme. Binding of the coenzyme is not affected by the Glu-67, substitution. A partial displacement of the active-site zinc in the, FDH.ADP-ribose binary complex indicates that the disruption of the, interaction between Glu-67 and Arg-368 is involved in the displacement of, active-site zinc. Kinetic studies with the R368L enzyme show that the, predominant role of Arg-368 is in the binding of the coenzyme. An, isomerization of the ternary complex before hydride transfer is detected, in the kinetic pathway of HMGSH. Steps involved in the binding of the, coenzyme to the FDH active site are also discerned from the unique, conformation of the coenzyme in one of the subunits of the E67L.NAD(H), binary complex.
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<StructureSection load='2fze' size='340' side='right'caption='[[2fze]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2fze]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FZE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FZE FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=APR:ADENOSINE-5-DIPHOSPHORIBOSE'>APR</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fze FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fze OCA], [https://pdbe.org/2fze PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fze RCSB], [https://www.ebi.ac.uk/pdbsum/2fze PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fze ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ADHX_HUMAN ADHX_HUMAN] Class-III ADH is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fz/2fze_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fze ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2FZE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with ZN, K, PO4 and APR as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2FZE OCA].
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*[[Alcohol dehydrogenase 3D structures|Alcohol dehydrogenase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structure-function relationships in human glutathione-dependent formaldehyde dehydrogenase. Role of Glu-67 and Arg-368 in the catalytic mechanism., Sanghani PC, Davis WI, Zhai L, Robinson H, Biochemistry. 2006 Apr 18;45(15):4819-30. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16605250 16605250]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Robinson, H.]]
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[[Category: Robinson H]]
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[[Category: Sanghani, P.C.]]
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[[Category: Sanghani PC]]
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[[Category: APR]]
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[[Category: K]]
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[[Category: PO4]]
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[[Category: ZN]]
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[[Category: adenosyl 5'-diphosphoribose]]
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[[Category: s-nitrosoglutathione reductase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 22:12:23 2007''
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Current revision

Crystal structure of the binary complex of human glutathione-dependent formaldehyde dehydrogenase with ADP-ribose

PDB ID 2fze

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