2chj

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[[Image:2chj.gif|left|200px]]
 
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==NMR structure of TGLGLT quadruplex==
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The line below this paragraph, containing "STRUCTURE_2chj", creates the "Structure Box" on the page.
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<StructureSection load='2chj' size='340' side='right'caption='[[2chj]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2chj]] is a 4 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CHJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CHJ FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LCG:[(1R,3R,4R,7S)-7-HYDROXY-3-(GUANIN-9-YL)-2,5-DIOXABICYCLO[2.2.1]HEPT-1-YL]METHYL+DIHYDROGEN+PHOSPHATE'>LCG</scene></td></tr>
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{{STRUCTURE_2chj| PDB=2chj | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2chj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2chj OCA], [https://pdbe.org/2chj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2chj RCSB], [https://www.ebi.ac.uk/pdbsum/2chj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2chj ProSAT]</span></td></tr>
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</table>
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'''NMR STRUCTURE OF TGLGLT QUADRUPLEX'''
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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==Overview==
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We have determined the NMR solution structures of the quadruplexes formed by d(TGLGLT) and d(TL4T), where L denotes LNA (locked nucleic acid) modified G-residues. Both structures are tetrameric, parallel and right-handed and the native global fold of the corresponding DNA quadruplex is retained upon introduction of the LNA nucleotides. However, local structural alterations are observed owing to the locked LNA sugars. In particular, a distinct change in the sugar-phosphate backbone is observed at the G2pL3 and L2pL3 base steps and sequence dependent changes in the twist between tetrads are also seen. Both the LNA modified quadruplexes have raised thermostability as compared to the DNA quadruplex. The quadruplex-forming capability of d(TGLGLT) is of particular interest as it expands the design flexibility for stable parallel LNA quadruplexes and shows that LNA nucleotides can be mixed with DNA or other modified nucleic acids. As such, LNA-based quadruplexes can be decorated by a variety of chemical modifications. Such LNA quadruplex scaffolds might find applications in the developing field of nanobiotechnology.
We have determined the NMR solution structures of the quadruplexes formed by d(TGLGLT) and d(TL4T), where L denotes LNA (locked nucleic acid) modified G-residues. Both structures are tetrameric, parallel and right-handed and the native global fold of the corresponding DNA quadruplex is retained upon introduction of the LNA nucleotides. However, local structural alterations are observed owing to the locked LNA sugars. In particular, a distinct change in the sugar-phosphate backbone is observed at the G2pL3 and L2pL3 base steps and sequence dependent changes in the twist between tetrads are also seen. Both the LNA modified quadruplexes have raised thermostability as compared to the DNA quadruplex. The quadruplex-forming capability of d(TGLGLT) is of particular interest as it expands the design flexibility for stable parallel LNA quadruplexes and shows that LNA nucleotides can be mixed with DNA or other modified nucleic acids. As such, LNA-based quadruplexes can be decorated by a variety of chemical modifications. Such LNA quadruplex scaffolds might find applications in the developing field of nanobiotechnology.
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==About this Structure==
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NMR solution structures of LNA (locked nucleic acid) modified quadruplexes.,Nielsen JT, Arar K, Petersen M Nucleic Acids Res. 2006 Apr 13;34(7):2006-14. Print 2006. PMID:16614450<ref>PMID:16614450</ref>
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2CHJ is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CHJ OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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NMR solution structures of LNA (locked nucleic acid) modified quadruplexes., Nielsen JT, Arar K, Petersen M, Nucleic Acids Res. 2006 Apr 13;34(7):2006-14. Print 2006. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16614450 16614450]
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</div>
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[[Category: Single protein]]
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<div class="pdbe-citations 2chj" style="background-color:#fffaf0;"></div>
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[[Category: Nielsen, J T.]]
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== References ==
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[[Category: Petersen, M.]]
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<references/>
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[[Category: Lna]]
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__TOC__
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[[Category: Locked nucleic acid]]
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</StructureSection>
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[[Category: Quadruplex]]
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[[Category: Large Structures]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 22:09:20 2008''
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[[Category: Nielsen JT]]
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[[Category: Petersen M]]

Current revision

NMR structure of TGLGLT quadruplex

PDB ID 2chj

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