2cny

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[[Image:2cny.gif|left|200px]]
 
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==Salmonella enterica SafA pilin in complex with a 19-residue SafA Nte peptide (I15A mutant)==
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The line below this paragraph, containing "STRUCTURE_2cny", creates the "Structure Box" on the page.
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<StructureSection load='2cny' size='340' side='right'caption='[[2cny]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2cny]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CNY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CNY FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cny FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cny OCA], [https://pdbe.org/2cny PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cny RCSB], [https://www.ebi.ac.uk/pdbsum/2cny PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cny ProSAT]</span></td></tr>
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{{STRUCTURE_2cny| PDB=2cny | SCENE= }}
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</table>
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== Function ==
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'''SALMONELLA ENTERICA SAFA PILIN IN COMPLEX WITH A 19-RESIDUE SAFA NTE PEPTIDE (I15A MUTANT)'''
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[https://www.uniprot.org/uniprot/Q8ZRK4_SALTY Q8ZRK4_SALTY]
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__TOC__
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</StructureSection>
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==Overview==
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[[Category: Large Structures]]
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Gram-negative pathogens commonly use the chaperone-usher pathway to assemble adhesive multisubunit fibers on their surface. In the periplasm, subunits are stabilized by a chaperone that donates a beta strand to complement the subunits' truncated immunoglobulin-like fold. Pilus assembly proceeds through a "donor-strand exchange" (DSE) mechanism whereby this complementary beta strand is replaced by the N-terminal extension (Nte) of an incoming pilus subunit. Using X-ray crystallography and real-time electrospray ionization mass spectrometry (ESI-MS), we demonstrate that DSE requires the formation of a transient ternary complex between the chaperone-subunit complex and the Nte of the next subunit to be assembled. The process is crucially dependent on an initiation site (the P5 pocket) needed to recruit the incoming Nte. The data also suggest a capping reaction displacing DSE toward product formation. These results support a zip-in-zip-out mechanism for DSE and a catalytic role for the usher, the molecular platform at which pili are assembled.
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[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
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[[Category: Ashcroft AE]]
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==About this Structure==
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[[Category: Hannan TJ]]
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2CNY is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CNY OCA].
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[[Category: Hultgren SJ]]
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[[Category: Radford SE]]
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==Reference==
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[[Category: Remaut H]]
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Donor-strand exchange in chaperone-assisted pilus assembly proceeds through a concerted beta strand displacement mechanism., Remaut H, Rose RJ, Hannan TJ, Hultgren SJ, Radford SE, Ashcroft AE, Waksman G, Mol Cell. 2006 Jun 23;22(6):831-42. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16793551 16793551]
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[[Category: Rose RJ]]
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[[Category: Protein complex]]
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[[Category: Waksman G]]
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[[Category: Salmonella typhimurium]]
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[[Category: Ashcroft, A E.]]
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[[Category: Hannan, T J.]]
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[[Category: Hultgren, S J.]]
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[[Category: Radford, S E.]]
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[[Category: Remaut, H.]]
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[[Category: Rose, R J.]]
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[[Category: Waksman, G.]]
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[[Category: Fibril protein]]
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[[Category: Fold complementation]]
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[[Category: Pilus subunit]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 22:37:35 2008''
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Current revision

Salmonella enterica SafA pilin in complex with a 19-residue SafA Nte peptide (I15A mutant)

PDB ID 2cny

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