2gli

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(New page: 200px<br /> <applet load="2gli" size="450" color="white" frame="true" align="right" spinBox="true" caption="2gli, resolution 2.600&Aring;" /> '''FIVE-FINGER GLI/DN...)
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[[Image:2gli.gif|left|200px]]<br />
 
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<applet load="2gli" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2gli, resolution 2.600&Aring;" />
 
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'''FIVE-FINGER GLI/DNA COMPLEX'''<br />
 
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==Overview==
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==FIVE-FINGER GLI/DNA COMPLEX==
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Zinc finger proteins, of the type first discovered in transcription factor, IIIA (TFIIIA), are one of the largest and most important families of, DNA-binding proteins. The crystal structure of a complex containing the, five Zn fingers from the human GLI oncogene and a high-affinity DNA, binding site has been determined at 2.6 A resolution. Finger one does not, contact the DNA. Fingers two through five bind in the major groove and, wrap around the DNA, but lack the simple, strictly periodic arrangement, observed in the Zif268 complex. Fingers four and five of GLI make, extensive base contacts in a conserved nine base-pair region, and this, section of the DNA has a conformation intermediate between B-DNA and, A-DNA. Analyzing the GLI complex and comparing it with Zif268 offers new, perspectives on Zn finger-DNA recognition.
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<StructureSection load='2gli' size='340' side='right'caption='[[2gli]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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== Structural highlights ==
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==Disease==
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<table><tr><td colspan='2'>[[2gli]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GLI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GLI FirstGlance]. <br>
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Known diseases associated with this structure: Holoprosencephaly-9 OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=165230 165230]], Pituitary anomalies with holoprosencephaly-like features OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=165230 165230]]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>
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==About this Structure==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gli OCA], [https://pdbe.org/2gli PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gli RCSB], [https://www.ebi.ac.uk/pdbsum/2gli PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gli ProSAT]</span></td></tr>
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2GLI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with CO as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2GLI OCA].
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</table>
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== Function ==
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==Reference==
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[https://www.uniprot.org/uniprot/GLI1_HUMAN GLI1_HUMAN] Acts as a transcriptional activator. May regulate the transcription of specific genes during normal development. May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling and thus cell proliferation and differentiation.<ref>PMID:10806483</ref> <ref>PMID:11238441</ref>
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Crystal structure of a five-finger GLI-DNA complex: new perspectives on zinc fingers., Pavletich NP, Pabo CO, Science. 1993 Sep 24;261(5129):1701-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8378770 8378770]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gl/2gli_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gli ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Pabo, C.O.]]
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[[Category: Pabo CO]]
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[[Category: Pavletich, N.P.]]
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[[Category: Pavletich NP]]
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[[Category: CO]]
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[[Category: protein/dna complex]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 22:19:58 2007''
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Current revision

FIVE-FINGER GLI/DNA COMPLEX

PDB ID 2gli

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