2d38

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[[Image:2d38.gif|left|200px]]
 
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==The Crystal Structure of Flavin Reductase HpaC complexed with NADP+==
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The line below this paragraph, containing "STRUCTURE_2d38", creates the "Structure Box" on the page.
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<StructureSection load='2d38' size='340' side='right'caption='[[2d38]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2d38]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfurisphaera_tokodaii_str._7 Sulfurisphaera tokodaii str. 7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D38 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2D38 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
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{{STRUCTURE_2d38| PDB=2d38 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2d38 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d38 OCA], [https://pdbe.org/2d38 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2d38 RCSB], [https://www.ebi.ac.uk/pdbsum/2d38 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2d38 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q974C9_SULTO Q974C9_SULTO]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d3/2d38_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2d38 ConSurf].
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<div style="clear:both"></div>
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'''The Crystal Structure of Flavin Reductase HpaC complexed with NADP+'''
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==See Also==
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*[[Flavin reductase|Flavin reductase]]
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__TOC__
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==Overview==
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</StructureSection>
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4-Hydroxyphenylacetate (4-HPA) is oxidized as an energy source by two component enzymes, the large component (HpaB) and the small component (HpaC). HpaB is a 4-HPA monooxygenase that utilizes FADH(2) supplied by a flavin reductase HpaC. We determined the crystal structure of HpaC (ST0723) from the aerobic thermoacidophilic crenarchaeon Sulfolobus tokodaii strain 7 in its three states [NAD(P)(+)-free, NAD(+)-bound, and NADP(+)-bound]. HpaC exists as a homodimer, and each monomer was found to contain an FMN. HpaC preferred FMN to FAD because there was not enough space to accommodate the AMP moiety of FAD in its flavin-binding site. The most striking difference between the NAD(P)(+)-free and the NAD(+)/NADP(+)-bound structures was observed in the N-terminal helix. The N-terminal helices in the NAD(+)/NADP(+)-bound structures rotated ca. 20 degrees relative to the NAD(P)(+)-free structure. The bound NAD(+) has a compact folded conformation with nearly parallel stacking rings of nicotinamide and adenine. The nicotinamide of NAD(+) stacked the isoalloxazine ring of FMN so that NADH could directly transfer hydride. The bound NADP(+) also had a compact conformation but was bound in a reverse direction, which was not suitable for hydride transfer.
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[[Category: Large Structures]]
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[[Category: Sulfurisphaera tokodaii str. 7]]
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==About this Structure==
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[[Category: Kamo M]]
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2D38 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_tokodaii_str._7 Sulfolobus tokodaii str. 7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D38 OCA].
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[[Category: Kudo N]]
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[[Category: Lee WC]]
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==Reference==
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[[Category: Nagata K]]
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Crystal structures of the short-chain flavin reductase HpaC from Sulfolobus tokodaii strain 7 in its three states: NAD(P)(+)(-)free, NAD(+)(-)bound, and NADP(+)(-)bound., Okai M, Kudo N, Lee WC, Kamo M, Nagata K, Tanokura M, Biochemistry. 2006 Apr 25;45(16):5103-10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16618099 16618099]
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[[Category: Okai M]]
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[[Category: Single protein]]
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[[Category: Tanokura M]]
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[[Category: Sulfolobus tokodaii str. 7]]
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[[Category: Kamo, M.]]
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[[Category: Kudo, N.]]
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[[Category: Lee, W C.]]
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[[Category: Nagata, K.]]
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[[Category: Okai, M.]]
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[[Category: Tanokura, M.]]
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[[Category: Flavin reductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 23:37:00 2008''
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Current revision

The Crystal Structure of Flavin Reductase HpaC complexed with NADP+

PDB ID 2d38

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