2h0d

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(New page: 200px<br /> <applet load="2h0d" size="450" color="white" frame="true" align="right" spinBox="true" caption="2h0d, resolution 2.50&Aring;" /> '''Structure of a Bmi-...)
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[[Image:2h0d.gif|left|200px]]<br />
 
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<applet load="2h0d" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2h0d, resolution 2.50&Aring;" />
 
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'''Structure of a Bmi-1-Ring1B Polycomb group ubiquitin ligase complex'''<br />
 
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==Overview==
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==Structure of a Bmi-1-Ring1B Polycomb group ubiquitin ligase complex==
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Polycomb group proteins Bmi-1 and Ring1B are core subunits of the PRC1, complex, which plays important roles in the regulation of Hox gene, expression, X-chromosome inactivation, tumorigenesis, and stem cell, self-renewal. The RING finger protein Ring1B is an E3 ligase that, participates in the ubiquitination of lysine 119 of histone H2A, and the, binding of Bmi-1 stimulates the E3 ligase activity. We have mapped the, regions of Bmi-1 and Ring1B required for efficient ubiquitin transfer and, determined a 2.5-A structure of the Bmi-1-Ring1B core domain complex. The, structure reveals that Ring1B "hugs" Bmi-1 through extensive RING domain, contacts and its N-terminal tail wraps around Bmi-1. The two regions of, interaction have a synergistic effect on the E3 ligase activity. Our, analyses suggest a model where the Bmi-1-Ring1B complex stabilizes the, interaction between the E2 enzyme and the nucleosomal substrate to allow, efficient ubiquitin transfer.
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<StructureSection load='2h0d' size='340' side='right'caption='[[2h0d]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2h0d]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H0D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2H0D FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2h0d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h0d OCA], [https://pdbe.org/2h0d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2h0d RCSB], [https://www.ebi.ac.uk/pdbsum/2h0d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2h0d ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/BMI1_HUMAN BMI1_HUMAN] Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.<ref>PMID:16359901</ref> <ref>PMID:16882984</ref> <ref>PMID:16714294</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h0/2h0d_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2h0d ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2H0D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2H0D OCA].
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*[[Polycomb complex protein|Polycomb complex protein]]
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*[[Ubiquitin protein ligase 3D structures|Ubiquitin protein ligase 3D structures]]
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==Reference==
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== References ==
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Structure of a Bmi-1-Ring1B polycomb group ubiquitin ligase complex., Li Z, Cao R, Wang M, Myers MP, Zhang Y, Xu RM, J Biol Chem. 2006 Jul 21;281(29):20643-9. Epub 2006 May 18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16714294 16714294]
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Xu, R.M.]]
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[[Category: Xu RM]]
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[[Category: ZN]]
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[[Category: chromatin]]
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[[Category: histone]]
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[[Category: polycomb]]
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[[Category: transcription]]
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[[Category: ubiquitin ligase]]
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[[Category: wpigenetics]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 22:24:29 2007''
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Current revision

Structure of a Bmi-1-Ring1B Polycomb group ubiquitin ligase complex

PDB ID 2h0d

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