2dr6

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[[Image:2dr6.gif|left|200px]]
 
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==Crystal structure of a multidrug transporter reveal a functionally rotating mechanism==
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The line below this paragraph, containing "STRUCTURE_2dr6", creates the "Structure Box" on the page.
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<StructureSection load='2dr6' size='340' side='right'caption='[[2dr6]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2dr6]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DR6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2DR6 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DM2:DOXORUBICIN'>DM2</scene></td></tr>
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{{STRUCTURE_2dr6| PDB=2dr6 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2dr6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dr6 OCA], [https://pdbe.org/2dr6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2dr6 RCSB], [https://www.ebi.ac.uk/pdbsum/2dr6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2dr6 ProSAT]</span></td></tr>
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</table>
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'''Crystal structure of a multidrug transporter reveal a functionally rotating mechanism'''
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== Function ==
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[https://www.uniprot.org/uniprot/ACRB_ECOLI ACRB_ECOLI] AcrAB is a drug efflux protein with a broad substrate specificity.<ref>PMID:16915237</ref> <ref>PMID:16946072</ref> <ref>PMID:17194213</ref>
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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AcrB is a principal multidrug efflux transporter in Escherichia coli that cooperates with an outer-membrane channel, TolC, and a membrane-fusion protein, AcrA. Here we describe crystal structures of AcrB with and without substrates. The AcrB-drug complex consists of three protomers, each of which has a different conformation corresponding to one of the three functional states of the transport cycle. Bound substrate was found in the periplasmic domain of one of the three protomers. The voluminous binding pocket is aromatic and allows multi-site binding. The structures indicate that drugs are exported by a three-step functionally rotating mechanism in which substrates undergo ordered binding change.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dr/2dr6_consurf.spt"</scriptWhenChecked>
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2DR6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DR6 OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Crystal structures of a multidrug transporter reveal a functionally rotating mechanism., Murakami S, Nakashima R, Yamashita E, Matsumoto T, Yamaguchi A, Nature. 2006 Sep 14;443(7108):173-9. Epub 2006 Aug 16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16915237 16915237]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2dr6 ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Matsumoto, T.]]
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[[Category: Matsumoto T]]
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[[Category: Murakami, S.]]
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[[Category: Murakami S]]
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[[Category: Nakashima, R.]]
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[[Category: Nakashima R]]
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[[Category: Yamashita, E.]]
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[[Category: Yamashita E]]
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[[Category: Antiporter]]
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[[Category: Drug resistance]]
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[[Category: Exporter]]
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[[Category: Membrane protein]]
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[[Category: Membrane transporter]]
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[[Category: Multidrug efflux]]
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[[Category: Transporter]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 00:59:47 2008''
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Current revision

Crystal structure of a multidrug transporter reveal a functionally rotating mechanism

PDB ID 2dr6

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