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2hfg

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(New page: 200px<br /> <applet load="2hfg" size="450" color="white" frame="true" align="right" spinBox="true" caption="2hfg, resolution 2.61&Aring;" /> '''Crystal structure o...)
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[[Image:2hfg.gif|left|200px]]<br />
 
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<applet load="2hfg" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2hfg, resolution 2.61&Aring;" />
 
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'''Crystal structure of hBR3 bound to CB3s-Fab'''<br />
 
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==Overview==
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==Crystal structure of hBR3 bound to CB3s-Fab==
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BR3, which is expressed on all mature B cells, is a specific receptor for, the B-cell survival and maturation factor BAFF (B-cell-activating factor, belonging to the tumor necrosis factor [TNF] family). In order to, investigate the consequences of targeting BR3 in murine models and to, assess the potential of BR3 antibodies as human therapeutics, synthetic, antibody phage libraries were employed to identify BAFF-blocking, antibodies cross-reactive to murine and human BR3, which share 52%, identity in their extracellular domains. We found an antibody, CB1, which, exhibits muM affinity for murine BR3 and very weak affinity for the human, receptor. CB3s, an affinity-matured variant of CB1, has sub-nM affinity, for BR3 from both species. Alanine scanning and crystallographic, structural analysis of the CB3s/BR3 complex reveal that CB3s mimics BAFF, by interacting with a similar region of the BR3 surface. Despite this, similarity in binding epitopes, CB1 variants antagonize BAFF-dependent, human B-cell proliferation in vitro and are effective at reducing murine, B-cell populations in vivo, showing significant promise as therapeutics, for human B-cell-mediated diseases.
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<StructureSection load='2hfg' size='340' side='right'caption='[[2hfg]], [[Resolution|resolution]] 2.61&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2hfg]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HFG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HFG FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.61&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hfg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hfg OCA], [https://pdbe.org/2hfg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hfg RCSB], [https://www.ebi.ac.uk/pdbsum/2hfg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hfg ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q6N093_HUMAN Q6N093_HUMAN]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hf/2hfg_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hfg ConSurf].
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<div style="clear:both"></div>
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==Disease==
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==See Also==
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Known disease associated with this structure: Kappa light chain deficiency OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=147200 147200]]
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*[[Antibody 3D structures|Antibody 3D structures]]
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*[[Tumor necrosis factor receptor 3D structures|Tumor necrosis factor receptor 3D structures]]
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==About this Structure==
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*[[3D structures of human antibody|3D structures of human antibody]]
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2HFG is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2HFG OCA].
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__TOC__
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</StructureSection>
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==Reference==
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Synthetic anti-BR3 antibodies that mimic BAFF binding and target both human and murine B cells., Lee CV, Hymowitz SG, Wallweber HJ, Gordon NC, Billeci KL, Tsai SP, Compaan DM, Yin J, Gong Q, Kelley RF, DeForge LE, Martin F, Starovasnik MA, Fuh G, Blood. 2006 Nov 1;108(9):3103-11. Epub 2006 Jul 13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16840730 16840730]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Hymowitz, S.G.]]
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[[Category: Hymowitz SG]]
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[[Category: antibody-receptor complex]]
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[[Category: crd]]
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[[Category: fab fragment]]
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[[Category: tnfrsf]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 22:31:53 2007''
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Current revision

Crystal structure of hBR3 bound to CB3s-Fab

PDB ID 2hfg

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