This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2e3w

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:38, 23 August 2023) (edit) (undo)
 
(12 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2e3w.gif|left|200px]]
 
-
<!--
+
==X-ray structure of native RNase A==
-
The line below this paragraph, containing "STRUCTURE_2e3w", creates the "Structure Box" on the page.
+
<StructureSection load='2e3w' size='340' side='right'caption='[[2e3w]], [[Resolution|resolution]] 1.05&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2e3w]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E3W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2E3W FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.05&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-
{{STRUCTURE_2e3w| PDB=2e3w | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2e3w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2e3w OCA], [https://pdbe.org/2e3w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2e3w RCSB], [https://www.ebi.ac.uk/pdbsum/2e3w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2e3w ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/RNAS1_BOVIN RNAS1_BOVIN] Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA.<ref>PMID:7479688</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e3/2e3w_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2e3w ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The total chemical synthesis of RNase A using modern chemical ligation methods is described, illustrating the significant advances that have been made in chemical protein synthesis since Gutte and Merrifield's pioneering preparation of RNase A in 1969. The identity of the synthetic product was confirmed through rigorous characterization, including the determination of the X-ray crystal structure to 1.1 Angstrom resolution.
-
'''X-ray structure of native RNase A'''
+
Total synthesis by modern chemical ligation methods and high resolution (1.1 A) X-ray structure of ribonuclease A.,Boerema DJ, Tereshko VA, Kent SB Biopolymers. 2008;90(3):278-86. PMID:17610259<ref>PMID:17610259</ref>
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 2e3w" style="background-color:#fffaf0;"></div>
-
==Overview==
+
==See Also==
-
The total chemical synthesis of RNase A using modern chemical ligation methods is described, illustrating the significant advances that have been made in chemical protein synthesis since Gutte and Merrifield's pioneering preparation of RNase A in 1969. The identity of the synthetic product was confirmed through rigorous characterization, including the determination of the X-ray crystal structure to 1.1 Angstrom resolution. (c) 2007 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 90:278-286, 2008.This article was originally published online as an accepted preprint. The "Published Online" date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com.
+
*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
-
 
+
== References ==
-
==About this Structure==
+
<references/>
-
2E3W is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E3W OCA].
+
__TOC__
-
 
+
</StructureSection>
-
==Reference==
+
-
Total synthesis by modern chemical ligation methods and high resolution (1.1 A) X-ray structure of ribonuclease A., Boerema DJ, Tereshko VA, Kent SB, Biopolymers. 2008;90(3):278-86. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17610259 17610259]
+
[[Category: Bos taurus]]
[[Category: Bos taurus]]
-
[[Category: Pancreatic ribonuclease]]
+
[[Category: Large Structures]]
-
[[Category: Single protein]]
+
[[Category: Boerema DJ]]
-
[[Category: Boerema, D J.]]
+
[[Category: Kent SBH]]
-
[[Category: Kent, S B.H.]]
+
[[Category: Tereshko VA]]
-
[[Category: Tereshko, V A.]]
+
-
[[Category: Alpha-helix]]
+
-
[[Category: Beta-sheet]]
+
-
[[Category: Hydrolase]]
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 01:52:38 2008''
+

Current revision

X-ray structure of native RNase A

PDB ID 2e3w

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2e3w"
Personal tools